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LASS6 Antibody

LASS6 Antibody

Item no.	PRS-4941-0.02mg
Manufacturer	ProSci
Amount	0.02 mg
Category	Metabolism Cholesterol & Lipid Metabolism Antibodies Immunohistochemistry Western Blot ELISA Immunofluorescence Enzymes Other Fatty Acid Enzymes Primary Antibodies
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IF, ELISA, IHC-P
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	LASS6 Antibody: CERS5, LASS6, Ceramide synthase 6, LAG1 longevity assurance homolog 6, CerS6
Available
Shipping	blue ice or RT
By Research Area	Homeostasis
Immunogen	LASS6 antibody was raised against a 13 amino acid synthetic peptide near the carboxy terminus of the human LASS6. The immunogen is located within the last 50 amino acids of LASS6.
Applications	LASS6 antibody can be used for detection of LASS6 by Western blot at 1 - 2 μ g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ g/mL. For immunofluorescence start at 20 μ g/mL. Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity	At least two isoforms of LASS6 are known to exist. This antibody is predicted not to cross-react with LASS5.
Positive Control 1	Cat. No. 1463 - Rat Brain Tissue Lysate
Purification	LASS6 Antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	LASS6 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	LASS6 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Related Products	Cat. No. 4941P – LASS6 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	CERS6
Accession #	AAI09285
Protein Gi #	80478334
Ncbi Gene Id #	253782
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	CERS6
Ncbi Official Full Name	ceramide synthase 6
Ncbi Organism	Homo sapiens
Swissprot #	Q6ZMG9
Background	LASS6 Antibody: The LASS (longevity assurance homolog) family members represent a subgroup of the homeobox gene family and are highly conserved from yeasts to mammals. Six members of this family of proteins have been characterized (LASS1-6) and all are involved in ceramide synthesis during cell growth regulation and cancer differentiation. Like the highly homologous LASS5, LASS6 is also an endoplasmic reticulum, multi-pass membrane protein. LASS6 is also involved in the synthesis of C14, C16 and C18-ceramide, but shows a preference for unsaturated fatty acids. LASS6 is broadly expressed in a wide range of tissues and microarray data suggests that it may play a role in cancer differentiation and early embryonic development.
Background References 1	Riebeling C, Allegood JC, Wang E, et al. Two mammalian longevity assurance gene (LAG1) family members, Trh1 and Trh, regulate dihydroceramide synthesis using different fatty acyl-CoA donors. J. Biol. Chem.2003; 278:43452-9.
Background References 2	Mizutani Y, Kihara A and Igarashi Y. Mammalian Lass6 and its related family members regulate synthesis of specific ceramides. Biochem. J.2005; 390:263-71.
Background References 3	Lahiri S and Futerman AH. LASS5 is a bona fide dihydroceramide synthase that selectively utilizes palmitoyl-CoA as acyl donor. J. Biol Chem.2005; 280:33735-8.
Background References 4	Weinmann A, Galle PR, and Teufel A. LASS6, an additional member of the longevity assurance gene family. Int. J. Mol. Med.2005; 16:905-10.
1st Image Caption	Western blot analysis of LASS6 in rat brain tissue lysate with LASS6 antibody at (A) 1 and (B) 2 μ g/mL.
2nd Image Caption	Immunohistochemistry of LASS6 in mouse brain tissue with LASS6 antibody at 2.5 μ g/mL.
3rd Image Caption	Immunofluorescence of LASS6 in Mouse Brain cells with LASS6 antibody at 5 μ g/mL.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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