ZBRK1 Antibody

blue ice or RT

ZBRK1 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of the human ZBRK1.

The immunogen is located within amino acids 410 - 460 of ZBRK1.

Cat. No. 1288 - A20 Cell Lysate

Polyclonal

Unconjugated

1 mg/mL

Cat. No. 4817P – ZBRK1 Peptide

None

NP_067645

59348

ZNF350

Homo sapiens

ZBRK1 Antibody: ZBRK1 is a member of the Kruppel-associated box-zinc finger protein (KRAB-ZFP) protein family of transcriptional repressors. ZBRK1 has been shown to not only be up-regulated in senescent fibroblasts but also interacts with BRCA1, a transcriptional regulator with tumor suppressor activity. ZBRK1 binds to a specific sequence within the GADD45 gene, supporting the assembly of a nuclear complex containing BRCA1 and repressing its transcription. Recent experiments demonstrate that ZBRK1 can homo-oligomerize in vitro and in vivo through a C-terminal transcriptional repression domain (CTRD), and this oligomerization facilitates the ZBRK1-directed transcriptional repression through ZBRK1 response elements. These results suggest that higher order ZBRK1 oligomers may assemble through both protein-DNA and CTRD-dependent protein-protein interactions.

Collins T, Stone JR, and Williams AJ. All in the family: the BTB/POZ, KRAB, and SCAN domains. Mol. Cell. Biol.2001; 21:3609-15.

Western blot analysis of ZBRK1 in A-20 lysate with ZBRK1 antibody at 1 μ g/mL in (A) the absence and (B) the presence of blocking peptide.