Syntaphilin Antibody

Neuroscience

Syntaphilin antibody can be used for detection of Syntaphilin by Western blot at 1 - 2 μ g/mL. Despite its predicted molecular weight, Syntaphilin usually migrates at higher molecular weight in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL.

Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Cat. No. 10-301 - Human Brain Tissue Slide

Polyclonal

Unconjugated

1 mg/mL

Cat. No. 4635P – Syntaphilin Peptide

None

O15079

9751

SNPH

Homo sapiens

Syntaphilin Antibody: Syntaphilin was initially identified in a yeast two-hybrid screen with the carboxy terminal region of Syntaxin-1 as bait. Syntaxin-1 is a key component of the synaptic vesicle docking machinery that forms the SNARE complex with synaptobrevin and SNAP-25. Syntaphilin competes with SNAP-25 for binding to syntaxin-1 and inhibits the formation of the SNARE complex, thereby potentially regulating synaptic vesicle exocytosis. Syntaphilin also binds dynamin-1 and inhibits dynamin-dependent endocytosis. Mice lacking syntaphilin show an increased level of mitochondrial motility and a reduced density of axonal mitochondria. This correlates with an enhanced short-term facilitation and significant impairments in motor ability, suggesting syntaphilin plays a major role in presynaptic function. Multiple isoforms are known to exist.

Sorensen JB. SNARE complexes prepare for membrane fusion. Trends Neurosci.2005; 28:453-5.

Kang J-S, Tian J-H, Pan P-Y, et al. Docking of axonal mitochondria by syntaphilin controls their mobility and affects short-term facilitation. Cell2008; 132:137-148.

Immunohistochemistry of Syntaphilin in human brain with Syntaphilin antibody at 5 μ g/mL.