Item no. |
PRS-4635-0.02mg |
Manufacturer |
ProSci
|
Amount |
0.02 mg |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IF, ELISA, IHC-P |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Syntaphilin Antibody: bA314N13.5, KIAA0374, Syntaphilin |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Neuroscience |
Immunogen |
Syntaphilin antibody was raised against a 16 amino acid synthetic peptide from near the center of human Syntaphilin.
The immunogen is located within amino acids 350 - 400 of Syntaphilin. |
Applications |
Syntaphilin antibody can be used for detection of Syntaphilin by Western blot at 1 - 2 μ g/mL. Despite its predicted molecular weight, Syntaphilin usually migrates at higher molecular weight in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Positive Control 1 |
Cat. No. 1463 - Rat Brain Tissue Lysate |
Positive Control 2 |
Cat. No. 10-301 - Human Brain Tissue Slide |
Purification |
Syntaphilin Antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
Syntaphilin Antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
Syntaphilin antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Related Products |
Cat. No. 4635P – Syntaphilin Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
SNPH |
Accession # |
O15079 |
Protein Gi # |
21362912 |
Ncbi Gene Id # |
9751 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
SNPH |
Ncbi Official Full Name |
syntaphilin |
Ncbi Organism |
Homo sapiens |
Swissprot # |
O15079 |
Background |
Syntaphilin Antibody: Syntaphilin was initially identified in a yeast two-hybrid screen with the carboxy terminal region of Syntaxin-1 as bait. Syntaxin-1 is a key component of the synaptic vesicle docking machinery that forms the SNARE complex with synaptobrevin and SNAP-25. Syntaphilin competes with SNAP-25 for binding to syntaxin-1 and inhibits the formation of the SNARE complex, thereby potentially regulating synaptic vesicle exocytosis. Syntaphilin also binds dynamin-1 and inhibits dynamin-dependent endocytosis. Mice lacking syntaphilin show an increased level of mitochondrial motility and a reduced density of axonal mitochondria. This correlates with an enhanced short-term facilitation and significant impairments in motor ability, suggesting syntaphilin plays a major role in presynaptic function. Multiple isoforms are known to exist. |
Background References 1 |
Lao G, Scheuss V, Gerwin CM, et al. Syntaphilin: a syntaxin-1 clamp that controls SNARE assembly. Neuron2000; 25:191-201. |
Background References 2 |
Sorensen JB. SNARE complexes prepare for membrane fusion. Trends Neurosci.2005; 28:453-5. |
Background References 3 |
Das S, Gerwin C, and Sheng ZH. Syntaphilin binds to dynamin-1 and inhibits dynamin-dependent endocytosis. J. Biol. Chem.2003; 278:41221-6. |
Background References 4 |
Kang J-S, Tian J-H, Pan P-Y, et al. Docking of axonal mitochondria by syntaphilin controls their mobility and affects short-term facilitation. Cell2008; 132:137-148. |
1st Image Caption |
Western blot analysis of Syntaphilin in rat brain tissue lysate with Syntaphilin antibody at (A) 1 and (B) 2 μ g/mL. |
2nd Image Caption |
Immunohistochemistry of Syntaphilin in human brain with Syntaphilin antibody at 5 μ g/mL. |
3rd Image Caption |
Immunofluorescence of Syntaphilin in Human Brain cells with Syntaphilin antibody at 20 μ g/mL. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.