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GPAT1 Antibody

GPAT1 Antibody

Item no.	PRS-4613-0.1mg
Manufacturer	ProSci
Amount	0.1 mg
Category	Metabolism Diabetes Cholesterol & Lipid Metabolism Regulation of Appetite Antibodies Immunohistochemistry Western Blot ELISA Immunofluorescence Primary Antibodies
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IF, ELISA, IHC-P
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	GPAT1 Antibody: GPAT, GPAT1, KIAA1560, Glycerol-3-phosphate acyltransferase 1, mitochondrial, GPAT-1
Available
Shipping	blue ice or RT
By Research Area	Obesity
Homology	Predicted species reactivity based on immunogen sequence: Pig: (100%), Bovine: (93%)
Immunogen	GPAT1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of the human GPAT1. The immunogen is located within amino acids 730 - 780 of GPAT1.
Applications	GPAT1 antibody can be used for detection of GPAT1 by Western blot at 1 - 2 μ g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ g/mL. For immunofluorescence start at 20 μ g/mL. Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
Specificity	At least four isoforms of GPAT1 are known to exist; this antibody will detect only the two largest isoforms.
Positive Control 1	Cat. No. 1463 - Rat Brain Tissue Lysate
Predicted Molecular Weight	Predicted: 87, 91 kDa Observed: 92 kDa
Mw Explanation	Lower molecular weight bands may represent alternate isoforms of GPAT1
Purification	GPAT1 Antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	GPAT1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	GPAT1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Related Products	Cat. No. 4613P – GPAT1 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	GPAM
Accession #	NP_065969
Protein Gi #	190358539
Ncbi Gene Id #	57678
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	GPAM
Ncbi Official Full Name	glycerol-3-phosphate acyltransferase, mitochondrial
Ncbi Organism	Homo sapiens
Swissprot #	Q9HCL2
Background	GPAT1 Antibody: Glycerol-3-phosphate acyltransferase 1 (GPAT1), one of four known GPAT isoforms, is located on the mitochondrial outer membrane, allowing reciprocal regulation with carnitine palmitoyltransferase-1. It is thought to be critical for the development of hepatic steatosis; steatosis triggered by GPAT1 overexpression leads to hepatic and possibly peripheral insulin resistance. GPAT1 is transcriptionally upregulated by insulin and sterol regulatory element binding protein (SREBP-1) and downregulated by AMP-activated protein kinase. Mice deficient in GPAT1 exhibit decreased triacylglycerol (TAG) in cardiomyocytes even in high-fat diets, suggesting that GPAT1 contributes significantly to TAG accumulation in heart tissue during lipogenic or high fat diets.
Background References 1	Coleman RA and Lee DP. Enzymes of triacylglycerol synthesis and their regulation. Prog. Lipid Res. 2004; 43:134-76.
Background References 2	Linden D, William-Olsson L, Ahnmark A, et al. Liver-directed overexpression of mitochondrial glycerol-3-phosphate acyltransferase results in hepatic steatosis, increased triacylglycerol secretion and reduced fatty acid oxidation. FASEB J. 2006; 20:434-43.
Background References 3	Eberle D, Hegarty B, Bossard P, et al. SREBP transcription factors: master regulators of lipid homeostasis. Biochimie 2004; 86:839-48.
Background References 4	Lewin TM, de Jong H, Schwerbrock NJ, et al. Mice deficient in glycerol-3-phosphate acyltransferase-1 have diminished myocardial triacylglycerol accumulation during lipogenic diet and altered phospholipid fatty acid composition. Biochim. Biophys. Acta 2008; 1781:352-8.
1st Image Caption	Western blot analysis of GPAT1 in rat brain tissue lysate with GPAT1 antibody at (A) 1 and (B) 2 μ g/mL.
2nd Image Caption	Immunohistochemistry of GPAT1 in rat brain with GPAT1 antibody at 2.5 μ g/mL.
3rd Image Caption	Immunofluorescence of GPAT1 in Rat Brain tissue with GPAT1 antibody at 20 μ g/mL.
4th Image Caption	Immunohistochemistry of GPAT1 in mouse brain tissue with GPAT1 antibody at 5 μ g/ml.
5th Image Caption	Immunofluorescence of GPAT1 in mouse brain tissue with GPAT1 antibody at 20 μ g/mL. Green: GPAT1 Antibody (4613) Blue: DAPI staining

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mg

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