Item no. |
PRS-46-892-0.1mg |
Manufacturer |
ProSci
|
Amount |
0.1 mg |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
IHC, ELISA |
Specific against |
Human |
Host |
Goat |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
RETNLB , resistin like beta , FIZZ1, FIZZ2, HXCP2, RELM-beta, RELMb, RELMbeta, XCP2, C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 2, colon and small intestine-specific cysteine-rich protein, cysteine-rich secreted A12 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer , Signal Transduction |
Immunogen |
The immunogen for this antibody is: C-DSVMDKKIKDVLNS |
Applications |
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 35kDa band in Human Ileum and Duodenum lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 11.7kDa according to NP_115968.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Colon shows textured staining of cytoplasm in enterocytes. Recommended concentration, 2-4ug/ml. |
Specificity |
. |
Positive Control 1 |
Cat. No. 1320 - Human Colon Tissue Lysate |
Purification |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing. |
Concentration |
500 ug/mL |
Storage Conditions |
Aliquot and store at -20˚ C. Minimize freezing and thawing. |
Related Products |
Cat. No. 46-892P - Resistin-like beta Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
RETNLB |
Accession # |
NP_115968.1 |
Protein Gi # |
14211897 |
Ncbi Gene Id # |
84666 |
Ncbi Official Symbol |
RETNLB |
Ncbi Official Full Name |
resistin like beta |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q9BQ08 |
Background References 1 |
McVay LD, Keilbaugh SA, Wong TM, Kierstein S, Shin ME, Lehrke M, Lefterova MI, Shifflett DE, Barnes SL, Cominelli F, Cohn SM, Hecht G, Lazar MA, Haczku A, Wu GD. Absence of bacterially induced RELMbeta reduces injury in the dextran sodium sulfate model of colitis. J Clin Invest. 2006 Nov; 116(11):2914-23. Epub 2006 Oct 5. |
1st Image Caption |
46-892 (2.5g/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.