Comparison

Resistin-like beta Antibody

Item no. PRS-46-892-0.1mg
Manufacturer ProSci
Amount 0.1 mg
Category
Type Antibody Primary
Format Liquid
Applications IHC, ELISA
Specific against Human
Host Goat
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias RETNLB , resistin like beta , FIZZ1, FIZZ2, HXCP2, RELM-beta, RELMb, RELMbeta, XCP2, C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 2, colon and small intestine-specific cysteine-rich protein, cysteine-rich secreted A12
Available
Shipping
blue ice or RT
By Research Area
Cancer , Signal Transduction
Immunogen
The immunogen for this antibody is: C-DSVMDKKIKDVLNS
Applications
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 35kDa band in Human Ileum and Duodenum lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 11.7kDa according to NP_115968.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Colon shows textured staining of cytoplasm in enterocytes. Recommended concentration, 2-4ug/ml.
Specificity
.
Positive Control 1
Cat. No. 1320 - Human Colon Tissue Lysate
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Conjugate
Unconjugated
Buffer
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration
500 ug/mL
Storage Conditions
Aliquot and store at -20˚ C. Minimize freezing and thawing.
Related Products
Cat. No. 46-892P - Resistin-like beta Peptide
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
RETNLB
Accession #
NP_115968.1
Protein Gi #
14211897
Ncbi Gene Id #
84666
Ncbi Official Symbol
RETNLB
Ncbi Official Full Name
resistin like beta
Ncbi Organism
Homo sapiens
Swissprot #
Q9BQ08
Background References 1
McVay LD, Keilbaugh SA, Wong TM, Kierstein S, Shin ME, Lehrke M, Lefterova MI, Shifflett DE, Barnes SL, Cominelli F, Cohn SM, Hecht G, Lazar MA, Haczku A, Wu GD. Absence of bacterially induced RELMbeta reduces injury in the dextran sodium sulfate model of colitis. J Clin Invest. 2006 Nov; 116(11):2914-23. Epub 2006 Oct 5.
1st Image Caption
46-892 (2.5g/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.1 mg
Available: In stock
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