Comparison

APOBEC2 Antibody

Item no. PRS-46-624-0.1mg
Manufacturer ProSci
Amount 0.1 mg
Category
Type Antibody Primary
Format Liquid
Applications WB, ELISA
Specific against Human
Host Goat
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias APOBEC2, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 2, ARCD1, ARP1, OTTHUMP00000039773, apolipoprotein B mRNA editing enzyme, catalytic polypeptide 2
Available
Shipping
blue ice or RT
By Research Area
Cancer , Signal Transduction
Homology
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen
The immunogen for this antibody is: C-EAASQNGEDLENLDD
Applications
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 26kDa band observed in Human Heart and Skeletal Muscle lysates (calculated MW of 25.7kDa according to NP_006780.1). Recommended concentration: 1-3ug/ml.An additional band of 90kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
Specificity
.
Positive Control 1
Cat. No. 1301 - Human Heart Tissue Lysate
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Conjugate
Unconjugated
Buffer
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration
500 ug/mL
Storage Conditions
Aliquot and store at -20˚ C. Minimize freezing and thawing.
Related Products
Cat. No. 46-624P - APOBEC2 Peptide
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
APOBEC2
Accession #
NP_006780.1
Protein Gi #
5802978
Ncbi Gene Id #
10930
Ncbi Official Symbol
APOBEC2
Ncbi Official Full Name
apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 2
Ncbi Organism
Homo sapiens
Swissprot #
Q9Y235
Background References 1
Prochnow C, Bransteitter R, Klein MG, Goodman MF, Chen XS. The APOBEC-2 crystal structure and functional implications for the deaminase AID. Nature. 2007 Jan 25; 445(7126):447-51. Epub 2006 Dec 24.
1st Image Caption
46-624 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.1 mg
Available: In stock
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