Item no. |
PRS-46-624-0.1mg |
Manufacturer |
ProSci
|
Amount |
0.1 mg |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, ELISA |
Specific against |
Human |
Host |
Goat |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
APOBEC2, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 2, ARCD1, ARP1, OTTHUMP00000039773, apolipoprotein B mRNA editing enzyme, catalytic polypeptide 2 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer , Signal Transduction |
Homology |
Expected Species Reactivity based on sequence homology: Mouse, Rat |
Immunogen |
The immunogen for this antibody is: C-EAASQNGEDLENLDD |
Applications |
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 26kDa band observed in Human Heart and Skeletal Muscle lysates (calculated MW of 25.7kDa according to NP_006780.1). Recommended concentration: 1-3ug/ml.An additional band of 90kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot. |
Specificity |
. |
Positive Control 1 |
Cat. No. 1301 - Human Heart Tissue Lysate |
Purification |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing. |
Concentration |
500 ug/mL |
Storage Conditions |
Aliquot and store at -20˚ C. Minimize freezing and thawing. |
Related Products |
Cat. No. 46-624P - APOBEC2 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
APOBEC2 |
Accession # |
NP_006780.1 |
Protein Gi # |
5802978 |
Ncbi Gene Id # |
10930 |
Ncbi Official Symbol |
APOBEC2 |
Ncbi Official Full Name |
apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 2 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q9Y235 |
Background References 1 |
Prochnow C, Bransteitter R, Klein MG, Goodman MF, Chen XS. The APOBEC-2 crystal structure and functional implications for the deaminase AID. Nature. 2007 Jan 25; 445(7126):447-51. Epub 2006 Dec 24. |
1st Image Caption |
46-624 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.