Item no. |
PRS-45-989-0.1mg |
Manufacturer |
ProSci
|
Amount |
0.1 mg |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, IHC, ELISA |
Specific against |
Human |
Host |
Goat |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
CREB3L1, OASIS, cAMP responsive element binding protein 3-like 1, BBF-2 (drosophila) homolog, old astrocyte specifically induced substance, BBF-2 homolog |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer |
Homology |
Expected Species Reactivity based on sequence homology: Mouse, Rat |
Immunogen |
The immunogen for this antibody is: C-DFSHSKEWFHDRD |
Applications |
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa band observed in lysates of cell line A549 (calculated MW of 57.0kDa according to NP_443086.1). Recommended concentration: 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle and Liver. Recommended concentration: 3.75ug/ml. |
Purification |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing. |
Concentration |
500 ug/mL |
Storage Conditions |
Aliquot and store at -20˚ C. Minimize freezing and thawing. |
Related Products |
Cat. No. 45-989P - OASIS Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
CREB3L1 |
Accession # |
NP_443086.1 |
Protein Gi # |
16418355 |
Ncbi Gene Id # |
90993 |
Ncbi Official Symbol |
CREB3L1 |
Ncbi Official Full Name |
cAMP responsive element binding protein 3-like 1 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q96BA8 |
Background References 1 |
Kondo S, Murakami T, Tatsumi K, Ogata M, Kanemoto S, Otori K, Iseki K, Wanaka A, Imaizumi K. OASIS, a CREB/ATF-family member, modulates UPR signalling in astrocytes. Nat Cell Biol. 2005 Feb; 7(2):186-94. Epub 2005 Jan 23. |
1st Image Caption |
45-989 (1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
2nd Image Caption |
45-989 (3.75ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
3rd Image Caption |
45-989 (3.75ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.