Item no. |
PRS-45-942-0.1mg |
Manufacturer |
ProSci
|
Amount |
0.1 mg |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
IHC, ELISA |
Specific against |
Human |
Host |
Goat |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
NEIL1, NEH1, nei endonuclease VIII-like 1 (E. coli), NEI1, FLJ22402, endonuclease VIII, FPG1, hFPG1, nei endonuclease VIII-like 1 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer |
Homology |
Expected Species Reactivity based on sequence homology: Pig, Cow |
Immunogen |
The immunogen for this antibody is: C-DIPSLEPEGTSAS |
Applications |
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 60kDa band in Human Liver and Brain lysates at 1ug/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 43.7kDda according to NP_078884.1. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Placenta shows strong staining of cytoplasm in syncytiotrophoblasts. Recommended concentration, 5-10ug/ml. |
Specificity |
. |
Positive Control 1 |
Cat. No. 1309 - Human Placenta Lysate |
Purification |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing. |
Concentration |
500 ug/mL |
Storage Conditions |
Aliquot and store at -20˚ C. Minimize freezing and thawing. |
Related Products |
Cat. No. 45-942P - NEIL1 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
NEIL1 |
Accession # |
NP_078884.2 |
Protein Gi # |
13375817 |
Ncbi Gene Id # |
79661 |
Ncbi Official Symbol |
NEIL1 |
Ncbi Official Full Name |
nei endonuclease VIII-like 1 (E. coli) |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q96FI4 |
Background References 1 |
Hazra TK, Izumi T, Boldogh I, Imhoff B, Kow YW, Jaruga P, Dizdaroglu M, Mitra S. Identification and characterization of a human DNA glycosylase for repair of modified bases in oxidatively damaged DNA. Proc Natl Acad Sci USA. 2002 Mar 19; 99(6):3523-8. |
1st Image Caption |
45-942 (5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.