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LASP1 Antibody

LASP1 Antibody

Item no.	PRS-45-823-0.1mg
Manufacturer	ProSci
Amount	0.1 mg
Category	Neuroscience Neural Lineage Markers Antibodies Western Blot ELISA Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	WB, IHC, ELISA
Specific against	Human
Host	Goat
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	LASP1, LIM and SH3 protein 1, Lasp-1, MLN50
Available
Shipping	blue ice or RT
By Research Area	Cancer
Homology	Expected Species Reactivity based on sequence homology: Dog
Immunogen	The immunogen for this antibody is: C-PERRDSQDGSSYR
Applications	Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 36kDa band observed in Human Kidney lysates (calculated MW of 29.7kDa according to NP_006139.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Schreiber et al, Gene. 1998 Jan 30; 207(2):171-5; PMID: 9511759). Recommended concentration: 0.03-0.1ug/ml.Immunohistochemistry:Paraffin embedded Human Colon and Small Intestine. Recommended concentration: 2.5ug/ml.
Specificity	.
Positive Control 1	Cat. No. 1305 - Human Kidney Tissue Lysate
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality	Polyclonal
Conjugate	Unconjugated
Buffer	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	500 ug/mL
Storage Conditions	Aliquot and store at -20˚ C. Minimize freezing and thawing.
Related Products	Cat. No. 45-823P - LASP1 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	LASP1
Accession #	NP_006139.1
Protein Gi #	5453710
Ncbi Gene Id #	3927
Ncbi Official Symbol	LASP1
Ncbi Official Full Name	LIM and SH3 protein 1
Ncbi Organism	Homo sapiens
Swissprot #	Q14847
Background References 1	Grunewald TG, Kammerer U, Schulze E, Schindler D, Honig A, Zimmer M, Butt E. Silencing of LASP-1 influences zyxin localization, inhibits proliferation and reduces migration in breast cancer cells. Exp Cell Res. 2006 Apr 15; 312(7):974-82. Epub 2006 Jan 23. Erratum in: Exp Cell
1st Image Caption	45-823 (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
2nd Image Caption	45-823 (2.5ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
3rd Image Caption	45-823 (2.5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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