Comparison

FOXP2 Antibody

Item no. PRS-45-625-0.1mg
Manufacturer ProSci
Amount 0.1 mg
Category
Type Antibody Primary
Format Liquid
Applications WB, IHC, ELISA
Specific against Human
Host Goat
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias FOXP2, forkhead box P2 , CAGH44, DKFZp686H1726, SPCH1, TNRC10 , CAG repeat protein 44, forkhead/winged-helix transcription factor, speech and language disorder 1, trinucleotide repeat containing 10
Available
Shipping
blue ice or RT
By Research Area
Cancer
Homology
Expected Species Reactivity based on sequence homology: Mouse, Cat, Dog, Pig, Cow, Zebrafish
Immunogen
The immunogen for this antibody is: C-DEVEYQKRRSQKIT
Applications
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 85kDa band observed in Human Brain (Cerebellum and Frontal Cortex) lysates and in Rat Brain lysates (calculated MW of 82.6kDa according to NP_683696.1). Recommended concentration: 0.3-1ug/ml.An additional band of unknown identity was also consistently observed at 28-30kDa. This band was successfully blocked by incubation with the immunising peptide. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Prostate and Placenta. Recommended concentration: 2.5ug/ml.
Specificity
This antibody is expected to recognize isoform I (NP_055306.1), isoform II (NP_683696.2), isoform IV (NP_683698.2) and isoform V (NP_001166237.1).
Positive Control 1
Cat. No. 1362 - Human Cerebellum, Left Lysate
Cat. No. 1363 - Human Cerebellum, Right Lysate
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Conjugate
Unconjugated
Buffer
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration
500 ug/mL
Storage Conditions
Aliquot and store at -20˚ C. Minimize freezing and thawing.
Related Products
Cat. No. 45-625P - FOXP2 Peptide
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
FOXP2
Accession #
NP_055306.1, NP_683696.2, NP_683697.2, NP_683698.2, NP_001166237.1
Protein Gi #
17017963
Ncbi Gene Id #
93986
Ncbi Official Symbol
FOXP2
Ncbi Official Full Name
forkhead box P2
Ncbi Organism
Homo sapiens
Swissprot #
O15409
Background References 1
Lai CS, Fisher SE, Hurst JA, Vargha-Khadem F, Monaco AP. A forkhead-domain gene is mutated in a severe speech and language disorder. Nature 2001 Oct 4; 413(6855):519-23
1st Image Caption
45-625 (0.3ug/ml) staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
2nd Image Caption
45-625 (2.5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
3rd Image Caption
45-625 (2.5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.1 mg
Available: In stock
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