Item no. |
PRS-45-625-0.1mg |
Manufacturer |
ProSci
|
Amount |
0.1 mg |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, IHC, ELISA |
Specific against |
Human |
Host |
Goat |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
FOXP2, forkhead box P2 , CAGH44, DKFZp686H1726, SPCH1, TNRC10 , CAG repeat protein 44, forkhead/winged-helix transcription factor, speech and language disorder 1, trinucleotide repeat containing 10 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer |
Homology |
Expected Species Reactivity based on sequence homology: Mouse, Cat, Dog, Pig, Cow, Zebrafish |
Immunogen |
The immunogen for this antibody is: C-DEVEYQKRRSQKIT |
Applications |
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 85kDa band observed in Human Brain (Cerebellum and Frontal Cortex) lysates and in Rat Brain lysates (calculated MW of 82.6kDa according to NP_683696.1). Recommended concentration: 0.3-1ug/ml.An additional band of unknown identity was also consistently observed at 28-30kDa. This band was successfully blocked by incubation with the immunising peptide. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Prostate and Placenta. Recommended concentration: 2.5ug/ml. |
Specificity |
This antibody is expected to recognize isoform I (NP_055306.1), isoform II (NP_683696.2), isoform IV (NP_683698.2) and isoform V (NP_001166237.1). |
Positive Control 1 |
Cat. No. 1362 - Human Cerebellum, Left Lysate Cat. No. 1363 - Human Cerebellum, Right Lysate |
Purification |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing. |
Concentration |
500 ug/mL |
Storage Conditions |
Aliquot and store at -20˚ C. Minimize freezing and thawing. |
Related Products |
Cat. No. 45-625P - FOXP2 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
FOXP2 |
Accession # |
NP_055306.1, NP_683696.2, NP_683697.2, NP_683698.2, NP_001166237.1 |
Protein Gi # |
17017963 |
Ncbi Gene Id # |
93986 |
Ncbi Official Symbol |
FOXP2 |
Ncbi Official Full Name |
forkhead box P2 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
O15409 |
Background References 1 |
Lai CS, Fisher SE, Hurst JA, Vargha-Khadem F, Monaco AP. A forkhead-domain gene is mutated in a severe speech and language disorder. Nature 2001 Oct 4; 413(6855):519-23 |
1st Image Caption |
45-625 (0.3ug/ml) staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence. |
2nd Image Caption |
45-625 (2.5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
3rd Image Caption |
45-625 (2.5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.