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Catalase Antibody

Catalase Antibody

Item no.	PRS-45-026-0.1mg
Manufacturer	ProSci
Amount	0.1 mg
Category	Antibodies Cell Biology Cell Status Immunohistochemistry Western Blot ELISA Oxidative Stress Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	WB, ELISA
Specific against	Human, Mouse
Host	Goat
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	CAT, catalase , HGNC:1516, MGC138422, MGC138424
Available
Shipping	blue ice or RT
By Research Area	Homeostasis
Homology	Expected Species Reactivity based on sequence homology: Dog
Immunogen	The immunogen for this antibody is: C-DKYNAEKPKNAIHT
Applications	Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 60kDa band observed in Human and Mouse Kidney and Liver lysates (calculated MW of 59.8kDa according to Human NP_001743.1 and Mouse NP_033934.2 ). Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature.
Specificity	.
Positive Control 1	Cat. No. 1305 - Human Kidney Tissue Lysate
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality	Polyclonal
Conjugate	Unconjugated
Buffer	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	500 ug/mL
Storage Conditions	Aliquot and store at -20˚ C. Minimize freezing and thawing.
Related Products	Cat. No. 45-026P - Catalase Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	CAT
Accession #	NP_001743.1
Protein Gi #	4557014
Ncbi Gene Id #	847
Ncbi Official Symbol	CAT
Ncbi Official Full Name	catalase
Ncbi Organism	Homo sapiens
Swissprot #	P04040
Background References 1	Schriner SE, Linford NJ, Martin GM, Treuting P, Ogburn CE, Emond M, Coskun PE, Ladiges W, Wolf N, Van Remmen H, Wallace DC, Rabinovitch PS. Extension of murine life span by overexpression of catalase targeted to mitochondria. Science. 2005 Jun 24; 308(5730):1909-11. Epub 2005 May 5.
1st Image Caption	45-026 (0.1ug/ml) staining of Human Kidney (A) and Liver (B) and Mouse Kidney (C) and Liver (D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
2nd Image Caption	45-026 (2ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
3rd Image Caption	45-026 (2ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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