Item no. |
PRS-45-026-0.1mg |
Manufacturer |
ProSci
|
Amount |
0.1 mg |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, ELISA |
Specific against |
Human, Mouse |
Host |
Goat |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
CAT, catalase , HGNC:1516, MGC138422, MGC138424 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Homeostasis |
Homology |
Expected Species Reactivity based on sequence homology: Dog |
Immunogen |
The immunogen for this antibody is: C-DKYNAEKPKNAIHT |
Applications |
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 60kDa band observed in Human and Mouse Kidney and Liver lysates (calculated MW of 59.8kDa according to Human NP_001743.1 and Mouse NP_033934.2 ). Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature. |
Specificity |
. |
Positive Control 1 |
Cat. No. 1305 - Human Kidney Tissue Lysate |
Purification |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing. |
Concentration |
500 ug/mL |
Storage Conditions |
Aliquot and store at -20˚ C. Minimize freezing and thawing. |
Related Products |
Cat. No. 45-026P - Catalase Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
CAT |
Accession # |
NP_001743.1 |
Protein Gi # |
4557014 |
Ncbi Gene Id # |
847 |
Ncbi Official Symbol |
CAT |
Ncbi Official Full Name |
catalase |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P04040 |
Background References 1 |
Schriner SE, Linford NJ, Martin GM, Treuting P, Ogburn CE, Emond M, Coskun PE, Ladiges W, Wolf N, Van Remmen H, Wallace DC, Rabinovitch PS. Extension of murine life span by overexpression of catalase targeted to mitochondria. Science. 2005 Jun 24; 308(5730):1909-11. Epub 2005 May 5. |
1st Image Caption |
45-026 (0.1ug/ml) staining of Human Kidney (A) and Liver (B) and Mouse Kidney (C) and Liver (D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
2nd Image Caption |
45-026 (2ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale. |
3rd Image Caption |
45-026 (2ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.