Item no. |
PRS-43-126-0.1mg |
Manufacturer |
ProSci
|
Amount |
0.1 mg |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IHC, ELISA |
Specific against |
Human |
Host |
Goat |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
CA1, carbonic anhydrase I, Car1, carbonic dehydratase |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer, Obesity, Signal Transduction, |
Homology |
Expected Species Reactivity based on sequence homology: Mouse, Rat |
Immunogen |
The immunogen for this antibody is: C-QAIKTKGKRAP |
Applications |
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 29kDa band observed in Human Liver lysates (calculated MW of 28.9kDa according to NP_001729.1. Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 2.5ug/ml. |
Specificity |
Reported variants represent identical protein: NP_001729.1, NP_001122303.1, NP_001122302.1, NP_001122301.1. |
Predicted Molecular Weight |
Approx 29kDa |
Purification |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing. |
Concentration |
500 ug/mL |
Storage Conditions |
Aliquot and store at -20˚ C. Minimize freezing and thawing. |
Related Products |
Cat. No. 43-126P - CA1 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
CA1 |
Accession # |
NP_001729.1 |
Protein Gi # |
4502517 |
Ncbi Gene Id # |
759 |
Ncbi Official Symbol |
CA1 |
Ncbi Official Full Name |
carbonic anhydrase I |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P00915 |
Background References 1 |
Gambhir KK, Ornasir J, Headings V, Bonar A, Decreased total carbonic anhydrase esterase activity and decreased levels of carbonic anhydrase 1 isozyme in erythrocytes of type II diabetic patients. |
1st Image Caption |
43-126 (0.03ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence. |
2nd Image Caption |
43-126 (2.5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.