Item no. |
PRS-42-921-0.1mg |
Manufacturer |
ProSci
|
Amount |
0.1 mg |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, ELISA |
Specific against |
Human, Mouse |
Host |
Goat |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
KLHDC8B, kelch domain containing 8B, FLJ11302, MGC35097, kelch domain-containing protein 8B |
Available |
|
Shipping |
blue ice or RT |
Homology |
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow |
Immunogen |
The immunogen for this antibody is: C-TKLPRSLRMRDK |
Applications |
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 38kDa band observed in lysates of cell lines Daudi, Jurkat and NIH3T3 (calculated MW of 37.7kDa according to NP_775817.1). Recommended concentration: 0.5-2ug/ml.An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? |
Specificity |
. |
Positive Control 1 |
Cat. No. 1224 - Daudi Cell Lysate |
Positive Control 2 |
Cat. No. 1205 - Jurkat Cell Lysate |
Predicted Molecular Weight |
Approx 38 kDa |
Purification |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing. |
Concentration |
500 ug/mL |
Storage Conditions |
Aliquot and store at -20˚ C. Minimize freezing and thawing. |
Related Products |
Cat. No. 42-921P - KLHDC8B Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
KLHDC8B |
Accession # |
NP_775817.1 |
Protein Gi # |
27734909 |
Ncbi Gene Id # |
200942 |
Ncbi Official Symbol |
KLHDC8B |
Ncbi Official Full Name |
kelch domain containing 8B |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q8IXV7 |
1st Image Caption |
42-921 (0.5ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.