Comparison

MTHFR Antibody

Item no. PRS-42-899-0.1mg
Manufacturer ProSci
Amount 0.1 mg
Category
Type Antibody Primary
Format Liquid
Applications WB, ELISA
Specific against Mouse
Host Goat
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias MTHFR, methylenetetrahydrofolate reductase (NAD(P)H), 5,10-methylenetetrahydrofolate reductase (NADPH), OTTHUMP00000002367, OTTHUMP00000002373, methylenetetrahydrofolate reductase
Available
Shipping
blue ice or RT
Homology
Expected Species Reactivity based on sequence homology: Human, Rat, Dog, Pig, Cow
Immunogen
The immunogen for this antibody is: C-SRPKSYIYRTQEWDE
Applications
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 70kDa band observed in lysates of cell line NIH3T3 (calculated MW of 74.6kDa according to Human NP_005948.3 and Mouse NP_034970.2). Recommended concentration: 0.3-1ug/ml.
Specificity
.
Positive Control 1
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Predicted Molecular Weight
Approx 70 kDa
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Conjugate
Unconjugated
Buffer
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration
500 ug/mL
Storage Conditions
Aliquot and store at -20˚ C. Minimize freezing and thawing.
Related Products
Cat. No. 42-899P - MTHFR Peptide
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
MTHFR
Accession #
NP_005948.3
Protein Gi #
87240000
Ncbi Gene Id #
4524
Ncbi Official Symbol
MTHFR
Ncbi Official Full Name
methylenetetrahydrofolate reductase (NAD(P)H)
Ncbi Organism
Homo sapiens
Swissprot #
P42898
1st Image Caption
42-899 (0.3ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.1 mg
Available: In stock
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