Comparison

Adam12 Antibody

Item no. PRS-42-860-0.1mg
Manufacturer ProSci
Amount 0.1 mg
Category
Type Antibody Primary
Format Liquid
Applications ELISA
Specific against other
Host Goat
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Adam12, a disintegrin and metallopeptidase domain 12 (meltrin alpha), KIAA4001, Mltna, mKIAA4001, ADAM 12, OTTMUSP00000023508, a disintegrin and metalloprotease domain 12, a disintegrin and metalloproteinase domain 12 (meltrin alpha), disintegrin and meta
Available
Shipping
blue ice or RT
Homology
Expected Species Reactivity based on sequence homology: Mouse, Rat, Pig
Immunogen
The immunogen for this antibody is: KKTTMEKLRCVHP
Applications
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Preliminary experiments gave an approx 37kDa band in Mouse and Rat Skeletal Muscle lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 98.5kDa according to NP_031426.2. The 37kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Specificity
This antibody is expected to recognize both reported isoforms (NP_003465.3; NP_067673.2).
Positive Control 1
Cat. No. 1407 - Mouse Skeletal Muscle Tissue Lysate
Positive Control 2
Cat. No. 1467 - Rat Skeletal Muscle Tissue Lysate
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Conjugate
Unconjugated
Buffer
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration
500 ug/mL
Storage Conditions
Aliquot and store at -20˚ C. Minimize freezing and thawing.
Related Products
Cat. No. 42-860P - Adam12 Peptide
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
Adam12
Accession #
NP_031426.2
Protein Gi #
117606341
Ncbi Gene Id #
8038
Ncbi Official Symbol
Adam12
Ncbi Official Full Name
ADAM metallopeptidase domain 12
Ncbi Organism
Homo sapiens
Swissprot #
O43184

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.1 mg
Available: In stock
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