Comparison

PTCHD1 Antibody

Item no. PRS-42-686-0.1mg
Manufacturer ProSci
Amount 0.1 mg
Category
Type Antibody Primary
Format Liquid
Applications WB, ELISA
Specific against Human
Host Goat
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias PTCHD1, patched domain containing 1, FLJ30296, MGC149798
Available
Shipping
blue ice or RT
By Research Area
Neuroscience
Homology
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen
The immunogen for this antibody is: C-DFQKTSRVSER
Applications
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 100kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 101kDa according to NP_775766.2). Recommended concentration: 0.5-2ug/ml.An additional band of unknown identity was also consistently observed at 50kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Specificity
.
Positive Control 1
Cat. No. 1362 - Human Cerebellum, Left Lysate
Cat. No. 1363 - Human Cerebellum, Right Lysate
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Conjugate
Unconjugated
Buffer
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration
500 ug/mL
Storage Conditions
Aliquot and store at -20˚ C. Minimize freezing and thawing.
Related Products
Cat. No. 42-686P - PTCHD1 Peptide
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
PTCHD1
Accession #
NP_775766.2
Protein Gi #
148271104
Ncbi Gene Id #
139411
Ncbi Official Symbol
PTCHD1
Ncbi Official Full Name
patched domain containing 1
Ncbi Organism
Homo sapiens
Swissprot #
Q96NR3
1st Image Caption
42-686 (0.5ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.1 mg
Available: In stock
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