Comparison

PSMB3 Antibody

Item no. PRS-42-647-0.1mg
Manufacturer ProSci
Amount 0.1 mg
Category
Type Antibody Primary
Format Liquid
Applications WB, IHC, ELISA
Specific against Human
Host Goat
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias PSMB3, proteasome (prosome, macropain) subunit, beta type, 3, HC10-II, MGC4147, proteasome beta 3 subunit, proteasome chain 13, proteasome component C10-II, proteasome theta chain
Available
Shipping
blue ice or RT
Homology
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow, Pig
Immunogen
The immunogen for this antibody is: C-NLYELKEGRQ
Applications
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 24kDa band observed in lysates of cell line K562 (calculated MW of 22.9kDa according to NP_002786.2). Recommended concentration: 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Lung. Recommended concentration: 10ug/ml.
Specificity
.
Positive Control 1
Cat. No. 1204 - K562 Cell Lysate
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Conjugate
Unconjugated
Buffer
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration
500 ug/mL
Storage Conditions
Aliquot and store at -20˚ C. Minimize freezing and thawing.
Related Products
Cat. No. 42-647P - PSMB3 Peptide
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
PSMB3
Accession #
NP_002786.2
Protein Gi #
22538465
Ncbi Gene Id #
5691
Ncbi Official Symbol
PSMB3
Ncbi Official Full Name
proteasome (prosome, macropain) subunit, beta type, 3
Ncbi Organism
Homo sapiens
Swissprot #
P49720
1st Image Caption
42-647 (1ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
2nd Image Caption
42-647 (10ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.1 mg
Available: In stock
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