Comparison

IDH1 Antibody

Item no. PRS-42-395-0.1mg
Manufacturer ProSci
Amount 0.1 mg
Category
Type Antibody Primary
Format Liquid
Applications WB, ELISA
Specific against other
Host Goat
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias IDH1, Idh1p, YNL037C, Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle
Available
Shipping
blue ice or RT
Immunogen
The immunogen for this antibody is: C-EPGSRHVGLDIKGQN
Applications
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 38kDa band observed in wildtype lysates of S.cerevisiae (calculated MW of 39.3kDa according to NP_014361.1). Recommended concentration: 0.3-1ug/ml.
Specificity
.
Positive Control 1
Wildtype lysates of S.cerevisiae
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Conjugate
Unconjugated
Buffer
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration
500 ug/mL
Storage Conditions
Aliquot and store at -20˚ C. Minimize freezing and thawing.
Related Products
Cat. No. 42-395P - IDH1 Peptide
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
IDH1
Accession #
NP_014361.1
Protein Gi #
6324291
Ncbi Gene Id #
855691
Ncbi Official Symbol
IDH1
Ncbi Official Full Name
isocitrate dehydrogenase (NAD(+)) IDH1
Ncbi Organism
Saccharomyces cerevisiae S288c
Swissprot #
P28834
1st Image Caption
42-395 (0.5ug/ml) staining of S. cerevisiae S288c lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. The blocking buffer contained non-animal derived protein. Data kindly provided by Dr. F Reggiori, Univer

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.1 mg
Available: In stock
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