Item no. |
PRS-42-395-0.1mg |
Manufacturer |
ProSci
|
Amount |
0.1 mg |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, ELISA |
Specific against |
other |
Host |
Goat |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
IDH1, Idh1p, YNL037C, Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle |
Available |
|
Shipping |
blue ice or RT |
Immunogen |
The immunogen for this antibody is: C-EPGSRHVGLDIKGQN |
Applications |
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 38kDa band observed in wildtype lysates of S.cerevisiae (calculated MW of 39.3kDa according to NP_014361.1). Recommended concentration: 0.3-1ug/ml. |
Specificity |
. |
Positive Control 1 |
Wildtype lysates of S.cerevisiae |
Purification |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing. |
Concentration |
500 ug/mL |
Storage Conditions |
Aliquot and store at -20˚ C. Minimize freezing and thawing. |
Related Products |
Cat. No. 42-395P - IDH1 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
IDH1 |
Accession # |
NP_014361.1 |
Protein Gi # |
6324291 |
Ncbi Gene Id # |
855691 |
Ncbi Official Symbol |
IDH1 |
Ncbi Official Full Name |
isocitrate dehydrogenase (NAD(+)) IDH1 |
Ncbi Organism |
Saccharomyces cerevisiae S288c |
Swissprot # |
P28834 |
1st Image Caption |
42-395 (0.5ug/ml) staining of S. cerevisiae S288c lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. The blocking buffer contained non-animal derived protein. Data kindly provided by Dr. F Reggiori, Univer |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.