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USP16 Antibody

USP16 Antibody

Item no.	PRS-42-222-0.1mg
Manufacturer	ProSci
Amount	0.1 mg
Category	Antibodies ELISA Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	ELISA
Specific against	other
Host	Goat
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	USP16, ubiquitin specific peptidase 16, UBP-M, deubiquitinating enzyme 16, ubiquitin carboxyl-terminal hydrolase 16, ubiquitin specific protease 16, ubiquitin thiolesterase 16, ubiquitin-specific processing protease 16
Available
Shipping	blue ice or RT
By Research Area	Homeostasis
Homology	Expected Species Reactivity based on sequence homology: Human, Mouse, Rat, Horse, Dog, Cow
Immunogen	The immunogen for this antibody is: NVEWNICQDCKTDNK
Applications	Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave bands at approx 50kDa and 26kDa in lysates of cell lines HEK293, A549 and HeLa after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 93.5kDa according to NP_006438.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Specificity	Reported variants represent identical protein: NP_001027582.1 NP_006438.1
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality	Polyclonal
Conjugate	Unconjugated
Buffer	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	500 ug/mL
Storage Conditions	Aliquot and store at -20˚ C. Minimize freezing and thawing.
Related Products	Cat. No. 42-222P - USP16 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	USP16
Accession #	NP_001027582.1, NP_001001992.1
Protein Gi #	75992941
Ncbi Gene Id #	10600
Ncbi Official Symbol	USP16
Ncbi Official Full Name	ubiquitin specific peptidase 16
Ncbi Organism	Homo sapiens
Swissprot #	Q9Y5T5
Background References 1	Joo HY, Zhai L, Yang C, Nie S, Erdjument-Bromage H, Tempst P, Chang C, Wang H Regulation of cell cycle progression and gene expression by H2A deubiquitination Nature. 2007 Oct 25; 449(7165):1068-72. Epub 2007 Oct 3

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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