Item no. |
PRS-42-222-0.1mg |
Manufacturer |
ProSci
|
Amount |
0.1 mg |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
ELISA |
Specific against |
other |
Host |
Goat |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
USP16, ubiquitin specific peptidase 16, UBP-M, deubiquitinating enzyme 16, ubiquitin carboxyl-terminal hydrolase 16, ubiquitin specific protease 16, ubiquitin thiolesterase 16, ubiquitin-specific processing protease 16 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Homeostasis |
Homology |
Expected Species Reactivity based on sequence homology: Human, Mouse, Rat, Horse, Dog, Cow |
Immunogen |
The immunogen for this antibody is: NVEWNICQDCKTDNK |
Applications |
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave bands at approx 50kDa and 26kDa in lysates of cell lines HEK293, A549 and HeLa after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 93.5kDa according to NP_006438.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? |
Specificity |
Reported variants represent identical protein: NP_001027582.1 NP_006438.1 |
Purification |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing. |
Concentration |
500 ug/mL |
Storage Conditions |
Aliquot and store at -20˚ C. Minimize freezing and thawing. |
Related Products |
Cat. No. 42-222P - USP16 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
USP16 |
Accession # |
NP_001027582.1, NP_001001992.1 |
Protein Gi # |
75992941 |
Ncbi Gene Id # |
10600 |
Ncbi Official Symbol |
USP16 |
Ncbi Official Full Name |
ubiquitin specific peptidase 16 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q9Y5T5 |
Background References 1 |
Joo HY, Zhai L, Yang C, Nie S, Erdjument-Bromage H, Tempst P, Chang C, Wang H Regulation of cell cycle progression and gene expression by H2A deubiquitination Nature. 2007 Oct 25; 449(7165):1068-72. Epub 2007 Oct 3 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.