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STIM2 Antibody

STIM2 Antibody

Item no.	PRS-4125-0.1mg
Manufacturer	ProSci
Amount	0.1 mg
Category	Antibodies Immunohistochemistry Western Blot ELISA Immunofluorescence Primary Antibodies
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IF, ELISA, IHC-P
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	STIM2 Antibody: KIAA1482, Stromal interaction molecule 2
Available
Shipping	blue ice or RT
By Research Area	Immunology
Immunogen	STIM2 antibody was raised against a 17 amino acid synthetic peptide from near the center of human STIM2. The immunogen is located within amino acids 140 - 190 of STIM2.
Applications	STIM2 antibody can be used for detection of STIM2 by Western blot at 0.5 - 2 μ g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ g/mL. For immunofluorescence start at 20 μ g/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1	Cat. No. 1288 - A20 Cell Lysate
Positive Control 2	Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 3	Cat. No. 10-901 - Human Spleen Tissue Slide
Purification	STIM2 Antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	STIM2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	STIM2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Related Products	Cat. No. 4125P – STIM2 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	STIM2
Accession #	NP_065911
Protein Gi #	41349446
Ncbi Gene Id #	57620
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	STIM2
Ncbi Official Full Name	stromal interaction molecule 2
Ncbi Organism	Homo sapiens
Swissprot #	Q9P246
Background	STIM2 Antibody: In T lymphocytes, the sole pathway for Ca++ entry following antigen-receptor binding is through store-operated Ca++-release-activated Ca++ (CRAC) channels. These channels are made up of the pore-forming subunit ORAI1 and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca++ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca++ store. A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca++ entry, and can form complexes with STIM1, suggesting they may play a coordinated role in controlling Ca++ entry. At least three isoforms of STIM2 are known to exist. This STIM2 antibody is predicted to have no cross-reactivity to STIM1.
Background References 1	Luik RM and Lewis RS. New insights into the molecular mechanisms of store-operated ^Ca2+ signaling in T cells. Trends Mol. Med.2007; 13:103-7.
Background References 2	Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature2006; 441:179-85.
Background References 3	Zhang SL, Yu Y, Roos J, et al. STIM1 is a Ca²⁺ sensor that activates CRAC channels and migrates from the Ca²⁺ store to the plasma membrane. Nature2005; 437:902-5.
Background References 4	Spassova MA, Soboloff J, He L-P, et al. STIM1 has a plasma membrane role in the activation of store-operated ^Ca2+ channels. Proc. Natl. Acad. Sci. USA2006; 103:4040-5.
1st Image Caption	Western blot analysis of STIM2 in A-20 cell lysate with STIM2 antibody at (A) 0.5, (B) 1 and (C) 2 μ g/mL.
2nd Image Caption	Immunohistochemistry of STIM2 in human spleen tissue with STIM2 antibody at 2.5 μ g/mL.
3rd Image Caption	Immunofluorescence of STIM2 in Human Spleen cells with STIM2 antibody at 20 μ g/mL.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mg

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