Item no. |
PRS-4013-0.02mg |
Manufacturer |
ProSci
|
Amount |
0.02 mg |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, ELISA, IHC-P |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
RPA Interacting Protein Antibody: RIP, HRIP, RIP, RPA-interacting protein, hRIP |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Homeostasis |
Immunogen |
RPA IP antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human RPA IP.
The immunogen is located within the last 50 amino acids of RPA Interacting Protein. |
Applications |
RPA Interacting Protein antibody can be used for detection of RPA Interacting Protein by Western blot at 1 - 2 μ g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ g/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. |
Positive Control 1 |
Cat. No. 1205 - Jurkat Cell Lysate |
Positive Control 2 |
Cat. No. 1415 - Mouse Stomach Tissue Lysate |
Purification |
RPA Interacting Protein Antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
RPA Interacting Protein Antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
RPA Interacting Protein antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Related Products |
Cat. No. 4013P – RPA Interacting Protein Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
RPAIN |
Accession # |
NP_001028174 |
Protein Gi # |
74136541 |
Ncbi Gene Id # |
84268 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
RPAIN |
Ncbi Official Full Name |
RPA interacting protein |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q86UA6 |
Background |
RPA Interacting Protein Antibody: Replication protein A (RPA) is a single-stranded-DNA binding protein involved in numerous eukaryotic DNA processes including replication, repair and recombination. RPA interacting protein (RPA IP) has been identified as an adapter protein that is involved in RPA nuclear import instead of the prototypical importin proteins that normally mediate nuclear import. Multiple isoforms of RPA IP are known to exist, with the longest isoform localized to the cytoplasm. Isoform 2 is sumoylated and is located in the PML nuclear body within the nucleus. It has been suggested that this isoform mediates the localization of the RPA complex into the PML nuclear body, thereby participating in RPA function in DNA metabolism. |
Background References 1 |
Zou Y, Liu Y, Wu X, et al. Functions of human replication protein A (RPA): from DNA replication to DNA damage and stress responses. J. Cell Physiol. 2006; 208:267-73. |
Background References 2 |
Jullien D, Gorlich D, Laemmli UK, et al. Nuclear import of RPA in Xenopus egg extracts requires a novel protein XRIPalpha but not importin α . EMBO J. 1999; 18:4348-58. |
Background References 3 |
Park J, Seo T, Kim H, et al. Sumoylation of the novel protein hRIPbeta is involved in replication protein A deposition in PML nuclear bodies. Mol. Cell Biol. 2005; 25:8202-14.(06-01D) |
1st Image Caption |
Western blot analysis of RPA Interacting Protein in Jurkat cell lysate with RPA Interacting Protein antibody at (A) 1 and (B) 2 μ g/mL. |
2nd Image Caption |
Immunohistochemistry of RPA Interacting Protein in mouse stomach tissue with RPA Interacting Protein antibody at 2.5 μ g/mL. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.