Item no. |
PRS-38-135-0.1mg |
Manufacturer |
ProSci
|
Amount |
0.1 mg |
Category |
|
Type |
Antibody Primary |
Format |
Lyophilized |
Applications |
WB, ELISA |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
OP-1, OP1, Bone morphogenetic protein 7, Osteogenic protein 1, BMP-7 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Growth Factor, Antibody Pairs, Functional Assays |
Immunogen |
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBMP-7. Human BMP-7 specific antibody was purified by affinity chromatography employing immobilized hBMP-7 matrix. |
Applications |
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human BMP-7 (100 ng/mL), a concentration of 0.4-0.6 μ g/mL of this antibody is required.
ELISA: Indirect: To detect hBMP-7 by indirect ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBMP-7.
Sandwich To detect hBMP-7 by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human BMP-7 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBMP-7.
Western Blot: To detect hBMP-7 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-7 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Storage Conditions |
BMP-7 antibody is stable for at least 2 years from date of receipt at -20˚ C. The reconstituted antibody is stable for at least two weeks at 2-8˚ C. Frozen aliquots are stable for at least 6 months when stored at -20˚ C. Avoid repeated freeze-thaw cycles. |
Disclaimer |
This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
BMP7 |
Accession # |
P18075 |
Protein Gi # |
115078 |
Ncbi Gene Id # |
655 |
User Note |
Centrifuge vial prior to opening. |
Ncbi Official Symbol |
BMP7 |
Ncbi Official Full Name |
bone morphogenetic protein 7 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P18075 |
Background |
BMPs (bone morphogenetic proteins) belong to the TGF beta superfamily of structurally related signaling proteins. Members of this superfamily are widely represented throughout the animal kingdom and have been implicated in a variety of developmental processes. Proteins of the TGF beta superfamily are disulfide-linked dimers composed of two 12-15 kDa polypeptide chains. As implied by their name, BMPs initiate, promote and regulate bone development, growth, remodeling and repair. |
1st Image Caption |
To detect hBMP-7 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human BMP-7 (38-136) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBMP-7. |
2nd Image Caption |
To detect hBMP-7 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-7 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
3rd Image Caption |
To detect hBMP-7 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-7 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
4th Image Caption |
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.25 ug/ml with an overnight incubation at 4˚ C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.