Item no. |
PRS-38-133-0.05mg |
Manufacturer |
ProSci
|
Amount |
0.05 mg |
Category |
|
Type |
Antibody Primary |
Format |
Lyophilized |
Applications |
WB, ELISA |
Specific against |
Human |
Host |
Goat |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
IL-20, IL10D, ZCYTO10, UNQ852/PRO1801, Interleukin-20, Cytokine Zcyto10, IL-20 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Immunology, Chemokines & Cytokines, Antibody Pairs |
Immunogen |
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hIL-20. Human IL-20 specific antibody was purified by affinity chromatography and then biotinylated. |
Applications |
ELISA:
Sandwich: To detect hIL-20 by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.25 - 1.0 μ g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human IL-20 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-20.
Western Blot: To detect hIL-20 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-20 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Clonality |
Polyclonal |
Conjugate |
Biotin |
Storage Conditions |
IL-20 antibody is stable for at least 2 years from date of receipt at -20˚ C. The reconstituted antibody is stable for at least two weeks at 2-8˚ C. Frozen aliquots are stable for at least 6 months when stored at -20˚ C. Avoid repeated freeze-thaw cycles. |
Disclaimer |
This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
IL20 |
Accession # |
Q9NYY1 |
Protein Gi # |
38258955 |
Ncbi Gene Id # |
50604 |
User Note |
Centrifuge vial prior to opening. |
Ncbi Official Symbol |
IL20 |
Ncbi Official Full Name |
interleukin 20 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q9NYY1 |
Background |
IL20 is a cytokine structurally related to interleukin 10 (IL10). This cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.