Comparison

NNT-1 Antibody (biotin)

Item no. PRS-38-131-0.05mg
Manufacturer ProSci
Amount 0.05 mg
Category
Type Antibody Primary
Format Lyophilized
Applications WB, ELISA
Specific against Human
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias CLC, NR6, BSF3, NNT1, BSF-3, CISS2, NNT-1, CLC, Cardiotrophin-like cytokine factor 1, B-cell-stimulating factor 3
Available
Shipping
blue ice or RT
By Research Area
Chemokines & Cytokines, Immunology, Antibody Pairs
Immunogen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hNNT-1/BCSF-3. Human NNT-1/BCSF-3 specific antibody was purified by affinity chromatography and then biotinylated.
Applications
ELISA:

Sandwich:
To detect hNNT-1 by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.25 - 1.0 μ g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human NNT-1 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNNT-1.

Western Blot:
To detect hNNT-1/BCSF-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNNT-1/BCSF-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Clonality
Polyclonal
Conjugate
Biotin
Storage Conditions
NNT-1 antibody is stable for at least 2 years from date of receipt at -20˚ C. The reconstituted antibody is stable for at least two weeks at 2-8˚ C. Frozen aliquots are stable for at least 6 months when stored at -20˚ C. Avoid repeated freeze-thaw cycles.
Disclaimer
This product is for research use only.
Modifications
None
Ncbi Official Symbol
CLCF1
Accession #
Q9UBD9
Protein Gi #
56404673
Ncbi Gene Id #
23529
User Note
Centrifuge vial prior to opening.
Ncbi Official Symbol
CLCF1
Ncbi Official Full Name
cardiotrophin-like cytokine factor 1
Ncbi Organism
Homo sapiens
Swissprot #
Q9UBD9
Background
NNT1 belongs to the interleukin 6 family of cytokines, which are involved in cell signaling through phosphorylation of gp130. IL6 family members share similarity in gene structure and have a 4 helix bundle in their protein structure.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.05 mg
Available: In stock
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