Item no. |
PRS-38-131-0.05mg |
Manufacturer |
ProSci
|
Amount |
0.05 mg |
Category |
|
Type |
Antibody Primary |
Format |
Lyophilized |
Applications |
WB, ELISA |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
CLC, NR6, BSF3, NNT1, BSF-3, CISS2, NNT-1, CLC, Cardiotrophin-like cytokine factor 1, B-cell-stimulating factor 3 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Chemokines & Cytokines, Immunology, Antibody Pairs |
Immunogen |
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hNNT-1/BCSF-3. Human NNT-1/BCSF-3 specific antibody was purified by affinity chromatography and then biotinylated. |
Applications |
ELISA:
Sandwich: To detect hNNT-1 by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.25 - 1.0 μ g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human NNT-1 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNNT-1.
Western Blot: To detect hNNT-1/BCSF-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNNT-1/BCSF-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Clonality |
Polyclonal |
Conjugate |
Biotin |
Storage Conditions |
NNT-1 antibody is stable for at least 2 years from date of receipt at -20˚ C. The reconstituted antibody is stable for at least two weeks at 2-8˚ C. Frozen aliquots are stable for at least 6 months when stored at -20˚ C. Avoid repeated freeze-thaw cycles. |
Disclaimer |
This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
CLCF1 |
Accession # |
Q9UBD9 |
Protein Gi # |
56404673 |
Ncbi Gene Id # |
23529 |
User Note |
Centrifuge vial prior to opening. |
Ncbi Official Symbol |
CLCF1 |
Ncbi Official Full Name |
cardiotrophin-like cytokine factor 1 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q9UBD9 |
Background |
NNT1 belongs to the interleukin 6 family of cytokines, which are involved in cell signaling through phosphorylation of gp130. IL6 family members share similarity in gene structure and have a 4 helix bundle in their protein structure. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.