Item no. |
PRS-3761-0.1mg |
Manufacturer |
ProSci
|
Amount |
0.1 mg |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IF, ELISA, IHC-P |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
LSD1 Antibody: AOF2, KDM1, LSD1, BHC110, AOF2, KIAA0601, Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Homeostasis |
Immunogen |
Anti-LSD1 antibody (3761) was raised against a peptide corresponding to 17 amino acids near the amino terminus of human LSD1.
The immunogen is located within amino acids 40-90 of LSD1. |
Applications |
WB: 0.2 - 2 μ g/mL; IHC-P: 2 μ g/mL; IF: 20 μ g/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry and Immunofluorescence in human samples. All other applications and species not yet tested. |
Positive Control 1 |
Cat. No. 1286 - P815 Cell Lysate |
Positive Control 2 |
Cat. No. 1224 - Daudi Cell Lysate |
Positive Control 3 |
Cat. No. 1205 - Jurkat Cell Lysate |
Positive Control 4 |
Cat. No. 1211 – HepG2 Cell Lysate |
Predicted Molecular Weight |
Predicted: 93kD
Observed: 110 kD |
Validation |
Independent Antibody Validation in Cell lines (Figure 2) shows similar LSD1 expression profile in human and mouse cell lines detected by two independent anti-LSD1 antibodies that recognize different epitopes, 3761 against N-terminus domain and 14-050 against recombinant fusion protein. LSD1 proteins are detected in the most tested cell lines that include mouse (3T3/NIH) and rat cell lines (YB2/0) at different expression levels by the two independent antibodies. |
Isoforms |
Human LSD1 has 2 isoforms, including isoform 1 (852aa, 93kD) and isoform 2 (876aa, 95kD). Mouse LSD1 has one isoform (853aa, 93kD) and Rat LSD1 also has one isoform (872aa, 95kD). 3761 can detect all isoforms of human, mouse and rat. |
Purification |
LSD1 Antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
LSD1 Antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
LSD1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Related Products |
Cat. No. 3761P – LSD1 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
KDM1 |
Accession # |
NP_001009999 |
Protein Gi # |
58761544 |
Ncbi Gene Id # |
23028 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
KDM1 |
Ncbi Official Full Name |
lysine (K)-specific demethylase 1 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
O60341 |
Background |
LSD1 Antibody: Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is a recently discovered amine oxidase that catalyzes the lysine-specific demethylation of histone proteins via an FAD-dependent oxidative reaction. Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues (such as H3-K4) is associated with active transcription. LSD1 associates with various proteins, including HDAC1/2, CoREST, and BHC80, that act to regulate LSD1 activity in vivo, and in a histone H3-K4-specific methylase complex that is involved in transcriptional regulation. Experiments have shown that CoREST, a SANT domain-containing corepressor acts to enhance LSD1 activity, while BHC80, a PHD domain-containing protein, inhibits CoREST/LSD1 activity in vitro. LSD1-mediated histone demethylation thus may have significant effects on gene expression. |
Background References 1 |
Shi et al. Cell 2004; 119:941-53. |
Background References 2 |
Kouzarides et al. Curr. Opin. Genet. Dev. 2002; 12:198-209. |
Background References 3 |
Shi et al. Mol. Cell 2005; 19:857-64. |
Background References 4 |
Nakamura et al. Mol. Cell 2002; 10:1119-28. |
1st Image Caption |
Figure 1 Western Blot Validation in Cell Lines Loading: 15 μ g of lysates per lane.Antibodies: LSD1 3761 (0.2 μ g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. |
2nd Image Caption |
Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines Loading: 15 μ g of lysates per lane.Antibodies: LSD1 3761 (0.2 μ g/mL), LSD1 14-050 (1 μ g/mL), beta-actin 3779 (1 μ g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. |
3rd Image Caption |
Figure 3 Western Blot Validation in Mouse Tissues Loading: 15 μ g of lysates per lane.Antibodies: LSD1 3761 (0.2 μ g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. |
4th Image Caption |
Figure 4 Western Blot Validation in Mouse P815 Cell Lines Loading: 15 μ g of lysates per lane.Antibodies: LSD1 3761, (A: 1 μ g/mL, B: 2 μ g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. |
5th Image Caption |
Figure 5 Immunohistochemistry Validation of LSD1 in Human Breast Carcinoma Tissue Immunohistochemical analysis of paraffin-embedded human breast carcinoma Tissue using anti-LSD1 antibody (3761) at 2 μ g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. |
6th Image Caption |
Figure 6 Immunofluorescence Validation of LSD1 in human breast carcinoma Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed human breast carcinoma Tissue labeling LSD1 with 3761 at 20 μ g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green/blue). |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.