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DDX3 Antibody

DDX3 Antibody

Item no.	PRS-3757-0.02mg
Manufacturer	ProSci
Amount	0.02 mg
Category	Oncology Neuroscience Targets for Rodent Neurosciences Antibodies By Cell Type Blastoma Western Blot ELISA Immunofluorescence Immunocytochemistry Primary Antibodies By Organ Hepatic Cancer
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IF, ICC, ELISA
Specific against	Human, Mouse
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	DDX3 Antibody: DBX, DDX3, HLP2, DDX14, DBX, ATP-dependent RNA helicase DDX3X, DEAD box protein 3, X-chromosomal
Available
Shipping	blue ice or RT
By Research Area	Infectious Disease
Immunogen	DDX3 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human DDX3. The immunogen is located within amino acids 110 - 160 of DDX3.
Applications	DDX3 antibody can be used for the detection of DDX3 by Western blot at 0.5 - 1 μ g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ g/mL. For immunofluorescence start at 20 μ g/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity	DDX3 antibody will detect both DDX3 and DBY.
Positive Control 1	Cat. No. 1211 - HepG2 Cell Lysate
Positive Control 2	Cat. No. 17-011 - Hep G2 Cell Slide
Purification	DDX3 Antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	DDX3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	DDX3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Related Products	Cat. No. 3757P – DDX3 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	DDX3X
Accession #	AAC34298
Protein Gi #	3523150
Ncbi Gene Id #	1654
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	DDX3X
Ncbi Official Full Name	DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
Ncbi Organism	Homo sapiens
Swissprot #	O00571
Background	DDX3 Antibody: DDX3 contains all of the motifs of the DEAD-box family of RNA helicases, including the Asp-Glu-Ala-Asp sequence that gives the protein family its name and distinguishes it from other RNA helicases. DDX3 is localized to the X chromosome and has a highly conserved functional homolog (DBY) on the Y chromosome. DDX3 is thought to be involved in RNA splicing, RNA transport, and translation initiation. It has also been shown to be involved in cell growth control and is deregulated in hepatitis virus-associated hepatocellular carcinoma. Recent experiments suppressing DDX3 expression blocked HIV-1 RNA export from the nucleus, suggesting that DDX3 functions as a shuttling protein that transports the HIV-1 protein Rev and its cofactor CRM1 from the nucleus to the cytoplasm.
Background References 1	Linder P, Lasko PF, Ashburner M, et al. Birth of the D-E-A-D box. Nature 1989; 337:121-2.
Background References 2	Park SH, Lee SG, Kim Y, et al. Assignment of a human putative RNA helicase gene, DDX3, to human X chromosome bands p11.3-->p11.23. Cytogenet. Cell Genet. 1998; 81:178-9.
Background References 3	Lahn BT and Page DC. Functional coherence of the human Y chromosome. Science 1997; 278:675-80.
Background References 4	Abdelhaleem M. RNA helicases: regulators of differentiation. Clin. Biochem. 2005; 38:499-503.
1st Image Caption	Western blot analysis of DDX3 in HepG2 cell lysate with DDX3 antibody at (A) 0.5 and (B) 1 μ g/mL.
2nd Image Caption	Immunocytochemistry of DDX3 in HepG2 cells with DDX3 antibody at 10 μ g/mL.
3rd Image Caption	Immunofluorescence of DDX3 in HepG2 cells with DDX3 antibody at 20 μ g/mL.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.02 mg

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Delivery expected until 9/6/2024