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TLR9 Antibody

TLR9 Antibody

Item no.	PRS-3739-0.1mg
Manufacturer	ProSci
Amount	0.1 mg
Category	Antibodies Western Blot ELISA Immunofluorescence Immunocytochemistry Primary Antibodies
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IF, ICC, ELISA
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	TLR9 Antibody: CD289, Toll-like receptor 9
Available
Shipping	blue ice or RT
By Research Area	Signal Transduction, Innate Immunity
Immunogen	TLR9 antibody was raised against a peptide corresponding to 15 amino acids near the center of human TLR9. The immunogen is located within amino acids 840 - 890 of TLR9.
Applications	TLR9 antibody can be used for detection of TLR9 by Western blot at 1 μ g/mL. Antibody can also be used for immunocytochemistry starting at 2 μ g/mL. For immunofluorescence start at 20 μ g/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity	At least two isoforms of TLR9 are known to exist; this antibody will detect both isoforms. TLR9 antibody is predicted to not cross-react with other TLR proteins.
Positive Control 1	Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 2	Cat. No. 17-005 - Jurkat Cell Slide
Predicted Molecular Weight	Predicted: 104, 111 kDa Observed: 97 kDa
Purification	TLR9 Antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	TLR9 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	TLR9 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Related Products	Cat. No. 3739P – TLR9 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	TLR9
Accession #	AAH32713
Protein Gi #	21595773
Ncbi Gene Id #	54106
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	TLR9
Ncbi Official Full Name	toll-like receptor 9
Ncbi Organism	Homo sapiens
Swissprot #	Q9NR96
Background	TLR9 Antibody: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. TLR9 forms a subfamily along with TLR7 and TLR8 that recognize viral RNA and CpG DNA sequences and are localized in intracellular acidic compartments such as the phagolysosome. Unlike other TLRs which act through adaptor molecules such as TOLLIP, TIRAP, TRIF, and MyD88 to activate various kinases and transcription factors to respond to potential infection, TLR9 is strictly dependent on MyD88.
Background References 1	Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol.2003; 21:335-76.
Background References 2	Janeway CA Jr. and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol.2002; 20:197-216.
Background References 3	Wagner H. The immunobiology of the TLR9 subfamily. Trends Immunol.2004; 381-6
Background References 4	Nishiya T and DeFranco AL. Ligand-regulated chimeric receptor approach reveals distinctive subcellular localization and signaling properties of the Toll-like receptors. J. Biol. Chem.2004; 279:19008-17.
1st Image Caption	Western blot analysis of TLR9 in Jurkat cell lysate with TLR9 antibody at 1 μ g/mL in (A) the absence and (B) the presence of blocking peptide
2nd Image Caption	Immunocytochemistry of TLR9 in Jurkat cells with TLR9 antibody at 2 μ g/mL.
3rd Image Caption	Immunofluorescence of TLR9 in Mouse Spleen tissue with TLR9 antibody at 10 μ g/mL.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mg

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Delivery expected until 10/4/2024

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