Item no. |
PRS-3607-0.1mg |
Manufacturer |
ProSci
|
Amount |
0.1 mg |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IF, ICC, ELISA |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
AES Antibody: GRG, ESP1, GRG5, TLE5, AES-1, AES-2, GRG, Amino-terminal enhancer of split, Gp130-associated protein GAM, Amino enhancer of split |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Apoptosis, Neuroscience |
Immunogen |
AES antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human AES.
The immunogen is located within the last 50 amino acids of AES. |
Applications |
AES antibody can be used for the detection of AES by Western blot at 1 - 4 μ g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Positive Control 1 |
Cat. No. 1210 - HEK293 Cell Lysate |
Positive Control 2 |
Cat. No. 1201 - HeLa Cell Lysate |
Positive Control 3 |
Cat. No. 17-001 - HeLa Cell Slide |
Purification |
AES Antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
AES Antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
AES antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Related Products |
Cat. No. 3607P – AES Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
AES |
Accession # |
NP_945320 |
Protein Gi # |
39812019 |
Ncbi Gene Id # |
166 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
AES |
Ncbi Official Full Name |
amino-terminal enhancer of split |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q08117 |
Background |
AES Antibody: Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis. It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin. |
Background References 1 |
Martin SS and Vuori K. Regulation of Bcl-2 proteins during anoikis and amorphosis. Biochim Biophys Acta. 2004; 1692:145-57. |
Background References 2 |
Miyasaka H, Choudhury BK, Hou WE, et al. Molecular cloning and expression of mouse and human cDNA encoding AES and ESG proteins with strong similarity to Drosophila enhancer of split groucho protein. Eur. J. Biochem. 1993; 216:343-52. |
Background References 3 |
Chen G and Courey AJ. Groucho/TLE family proteins and transcriptional repression. Gene 2000; 249:1-16. |
Background References 4 |
Jan Y, Matter M, Pai J-t, et al. A mitochondrial protein, Bit1, mediates apoptosis regulated by integrins and groucho/TLE corepressors. Cell 2004; 116:751-762. |
1st Image Caption |
Western blot analysis of AES in 293 cell lysate with AES antibody at (A) 1, (B) 2 and (C) 4 μ g/mL. |
2nd Image Caption |
Immunocytochemistry of AES in HeLa cells with AES antibody at 10 μ g/mL. |
3rd Image Caption |
Immunofluorescence of AES in Hela cells with AES antibody at 20 μ g/mL. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.