Item no. |
PRS-36-268-100ug |
Manufacturer |
ProSci
|
Amount |
100 ug |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, ELISA |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Angiopoietin-like Protein 4; FIAF; Fasting-induced Adipose Factor; HFARP; Hepatic Fibrinogen/Angiopoietin-related Protein |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer, Obesity |
Immunogen |
Fibrinogen-like domain of recombinant human ANGPTL4. |
Applications |
ELISA: (direct and indirect: 1:2, 000-1:5, 000). Western Blot: (1:2, 000-1:5, 000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application. |
Specificity |
Recognizes the fibrinogen-like domain of human ANGPTL4. Detects a band of ~38kDa by Western blot. Weakly cross-reacts with human ANGPTL3 (fibrinogen-like domain), ANGPTL5 (fibrinogen-like domain), ANGPTL6 (fibrinogen-like domain) and ANGPTL6. Does not cross-react with ANGPTL4 (coiled-coil domain) or other ANGPTL family proteins. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives. |
Concentration |
1 mg/ml |
Storage Conditions |
Stable for at least 6 months after receipt when stored at -20˚ C. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
ANGPTL4 |
Accession # |
25008123 |
Protein Gi # |
Q9BY76 |
Ncbi Gene Id # |
51129 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
ANGPTL4 |
Ncbi Official Full Name |
angiopoietin like 4 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q9BY76 |
Background |
ANGPTL4 mainly expressed in endothelial cells (hypoxia-induced). Regulates angiogenesis and modulates tumorgenesis and directly regulates lipid, glucose, and energy metabolism. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. ANGPTL4 is a protein consisting of an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain (FLD). Both domains have distinct biological functions. The coiled-coil domain is responsible for the inhibitory effects on lipoprotein lipase (LPL) converting the active form of LPL into an inactive form, and the FLD domain mediates its antiangiogenic functions. The coiled coil and the FLD domains are separated by a short linker that can be cleaved after secretion. ANGPTL4 appears on the cell surface as the full-length form, where it can be released by heparin treatment. ANGPTL4 protein is then proteolytically cleaved by proprotein convertases (PCs), including furin, PC5/6, paired basic amino acid-cleaving enzyme 4, and PC7. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.