« Back
Home /
p53 Antibody [SPM589]

p53 Antibody [SPM589]

Item no.	PRS-34-173-100ug
Manufacturer	ProSci
Amount	100 ug
Category	Oncology Food Safety Neuroscience Aging & Neurological Disorders Antibodies By Cell Type Hematology Leukemia Lymphoma Flow Cytometry Immunohistochemistry Western Blot Immunofluorescence Contaminants Primary Antibodies Metals By Organ Skin Cancer Renal Cancer
Type	Antibody Monoclonal
Format	Liquid
Applications	WB, FC, IF, IHC
Specific against	Human
Host	Mouse
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53, P53
Available
Shipping	blue ice or RT
By Research Area	Cancer, Cell Cycle, Signal Transduction,
Immunogen	Recombinant human wild type p53 protein was used as the immunogen for this anti-p53 antibody. Its epitope maps within the N-terminus (approx. amino acids 20-25).
Applications	Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Immunofluorescence: 1-2 ug/ml Western blot: 0.5-1 ug/ml Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1) Prediluted format: incubate for 30 min at RT (2) The optimal dilution of the anti-p53 antibody for each application should be determined by the researcher. 1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Specificity	Does not react with Mouse and Rat
Purification	Protein G affinity chromatography
Clonality	Monoclonal
Clone	SPM589
Isotype	IgG2a
Conjugate	Unconjugated
Buffer	PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration	0.2 mg/mL
Storage Conditions	Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	TP53
Ncbi Gene Id #	7157
User Note	Optimal dilutions for each application to be determined by the researcher
Ncbi Official Symbol	TP53
Ncbi Official Full Name	tumor protein p53
Ncbi Organism	Homo sapiens
Swissprot #	P04637
Background	p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis.
1st Image Caption	Formalin-fixed, paraffin-embedded human bladder carcinoma stained with anti-p53 antibody (SPM589).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Request product datasheet

Request safety datasheet

Simple Order Order Subscription

Amount: 100 ug

available

Delivery expected until 9/6/2024

Express Delivery: Save 2 days

Compare

Add to wishlist

Recommend by mail