Item no. |
PRS-34-133-100ug |
Manufacturer |
ProSci
|
Amount |
100 ug |
Category |
|
Type |
Antibody Monoclonal |
Format |
Liquid |
Applications |
FC, IF, IHC |
Specific against |
Human |
Host |
Mouse |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Alias |
MUC1 Antibody : EMA, MCD, PEM, PUM, KL-6, MAM6, MCKD, PEMT, CD227, H23AG, MCKD1, MUC-1, ADMCKD, ADMCKD1, CA 15-3, MUC-1/X, MUC1/ZD, MUC-1/SEC, Mucin-1, Breast carcinoma-associated antigen DF3 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Signal Transduction |
Immunogen |
Human milk fat globule membranes were used as the immunogen for this EMA antibody. This mAb reacts with the DTRP epitope in the tandem repeats. |
Applications |
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Immunofluorescence: 0.5-1 ug/ml Immunohistochemistry (FFPE): 0.1-0.2 ug/ml for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) The optimal dilution of the EMA antibody for each application should be determined by the researcher. 1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Purification |
Protein G affinity chromatography |
Clonality |
Monoclonal |
Clone |
SPM132 |
Isotype |
IgG1, kappa |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.1 mg/ml BSA and 0.05% sodium azide |
Concentration |
0.2 mg/mL |
Storage Conditions |
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
MUC1 |
Ncbi Gene Id # |
4582 |
User Note |
Optimal dilutions for each application to be determined by the researcher |
Ncbi Official Symbol |
MUC1 |
Ncbi Official Full Name |
mucin 1, cell surface associated |
Ncbi Organism |
homo sapiens |
Swissprot # |
P15941 |
Background |
Epithelial Marker Antigen is a 265-400kDa glycoprotein. The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver. |
1st Image Caption |
Formalin-fixed, paraffin-embedded human breast cancer stained with EMA antibody (SPM132). |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.