NADE Antibody

blue ice or RT

Predicted species reactivity based on immunogen sequence: Rat: (100%), Bovine: (100%)

NADE antibody can be used for detection of NADE by Western blot at 1 μ g/mL. Despite its predicted molecular weight, NADE migrates at ~23 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2 μ g/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.

Cat. No. 10-301 - Human Brain Tissue Slide

Polyclonal

Unconjugated

1 mg/mL

Cat. No. 3359P – NADE Peptide

None

NP_996798

27018

NGFRAP1

Homo sapiens

NADE Antibody: The p75 neurotrophin receptor (p75NTR) is a member of the tumor necrosis receptor superfamily and can mediate cell death and cell survival in response to nerve growth factor (NGF). The p75NTR-associated cell death executor (NADE) mediates apoptosis by interacting with the cell death domain of p75NTR following the binding of NGF by p75NTR. Recent studies have shown that NADE also interacts with second mitochondria-derived activator of caspase (Smac). Co-expression of NADE and Smac promotes TRAIL-induced apoptosis and inhibits XIAP-mediated Smac ubiquitization. It has been suggested that it is this interaction between NADE and Smac that allows apoptosis to proceed. Finally, although initially discovered as an mRNA expressed in ovarian granulosa cells, NADE has been suggested to play a role in the neuronal death seen in epileptic brain damage.

Mukai J, Hachiya T, Shoji-Hoshino S, et al. NADE, a p75NTR-associated cell death executor, is involved in signal transduction mediated by the common neurotrophin receptor p75NTR. J. Biol. Chem. 2000; 275:17566-70.

YI J-S, Lee, S-K, Sato T-A, et al. Co-induction of p75NTR and the associated death executor NADE in degenerating hippocampal neurons after kainate-induced seizures in the rat. Neurosci. Lett. 2003; 347:126-30.

Immunohistochemistry of NADE in human brain tissue with NADE antibody at 2 μ g/mL.