Item no. |
PRS-33-847-100ug |
Manufacturer |
ProSci
|
Amount |
100 ug |
Category |
|
Type |
Antibody Monoclonal |
Format |
Liquid |
Applications |
WB, FC, IF, IHC-P |
Specific against |
Human |
Host |
Mouse |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Alias |
DNA replication licensing factor MCM7, CDC47 homolog, P11-MCM3, MCM7, CDC47, MCM2 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Other |
Immunogen |
A human partial recombinant protein corresponding to amino acids 195-319 was used as the immunogen for the MCM7 antibody. |
Applications |
Immunofluorescence: 0.5-1 ug/ml Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT Prediluted IHC only format: incubate for 30 min at RT (1) Titering of the MCM7 antibody may be required for optimal performance. 1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Purification |
Protein G affinity chromatography |
Clonality |
Monoclonal |
Clone |
MCM7/1468 |
Isotype |
IgG2b |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.1 mg/ml BSA and 0.05% sodium azide |
Concentration |
0.2 mg/mL |
Storage Conditions |
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
MCM7 |
Ncbi Gene Id # |
4176 |
User Note |
Optimal dilutions for each application to be determined by the researcher |
Ncbi Official Symbol |
MCM7 |
Ncbi Official Full Name |
minichromosome maintenance complex component 7 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P33993 |
Background |
MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that is essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumor suppressor protein RB1/RB. |
1st Image Caption |
Analysis of HuProt(TM) microarray containing more than 19, 000 full-length human proteins using MCM7 antibody (clone MCM7/1468). Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target. |
2nd Image Caption |
IHC testing of FFPE human tonsil with MCM7 antibody (clone MCM7/1468). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min. |
3rd Image Caption |
SDS-PAGE Analysis of Purified, BSA-Free MCM7 Antibody (clone MCM7/1468). Confirmation of Integrity and Purity of the Antibody. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.