CELA3B Antibody [CELA3B/1757]

Cell Cycle, Signal Transduction

Western blot: 0.5-1 ug/ml

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

IF: 1-2 ug/ml

ELISA: 2-4 ug/ml; order BSA free formulation for coating

IHC (FFPE): 1-2 ug/ml for 30 min at RT

Optimal dilution of the CELA3B antibody should be determined by the researcher.

Monoclonal

IgG1, kappa

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

None

23436

CELA3B

Homo sapiens

This mAb recognizes a protein of ~30kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B), or Elastase 3B. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.

SDS-PAGE Analysis of Purified, BSA-Free CELA3B Antibody (clone CELA3B/1757). Confirmation of Integrity and Purity of the Antibody.