Comparison

CD86 Antibody [SPM600]

Item no. PRS-33-496-100ug
Manufacturer ProSci
Amount 100 ug
Category
Type Antibody Monoclonal
Format Liquid
Applications WB, FC, IF, IHC-P
Specific against Human, Mouse, Rat
Host Mouse
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B72, FUN-1, CD86, CD86, CD28LG2
Available
Shipping
blue ice or RT
By Research Area
Immunology, Signal Transduction
Immunogen
Recombinant human full-length protein was used as the immunogen for the CD86 antibody.
Applications
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 0.5-1 ug/ml

Western blot: 0.5-1 ug/ml

Immunohistochemistry (FFPE): 2-4 ug/ml for 30 min at RT (1)

Optimal dilution of the CD86 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.

Purification
Protein G affinity chromatography
Clonality
Monoclonal
Clone
SPM600
Isotype
IgG1, kappa
Conjugate
Unconjugated
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration
0.2 mg/mL
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
CD86
Ncbi Gene Id #
942
User Note
Optimal dilutions for each application to be determined by the researcher
Ncbi Official Symbol
CD86
Ncbi Official Full Name
CD86 molecule
Ncbi Organism
Homo sapiens
Swissprot #
P42081
Background
CD86 is a receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. [UniProt]
1st Image Caption
Formalin-fixed, paraffin-embedded human melanoma stained with CD86 antibody (SPM600).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
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