Item no. |
PRS-33-496-100ug |
Manufacturer |
ProSci
|
Amount |
100 ug |
Category |
|
Type |
Antibody Monoclonal |
Format |
Liquid |
Applications |
WB, FC, IF, IHC-P |
Specific against |
Human, Mouse, Rat |
Host |
Mouse |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Alias |
T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B72, FUN-1, CD86, CD86, CD28LG2 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Immunology, Signal Transduction |
Immunogen |
Recombinant human full-length protein was used as the immunogen for the CD86 antibody. |
Applications |
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Immunofluorescence: 0.5-1 ug/ml Western blot: 0.5-1 ug/ml Immunohistochemistry (FFPE): 2-4 ug/ml for 30 min at RT (1) Optimal dilution of the CD86 antibody should be determined by the researcher. 1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min. |
Purification |
Protein G affinity chromatography |
Clonality |
Monoclonal |
Clone |
SPM600 |
Isotype |
IgG1, kappa |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.1 mg/ml BSA and 0.05% sodium azide |
Concentration |
0.2 mg/mL |
Storage Conditions |
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
CD86 |
Ncbi Gene Id # |
942 |
User Note |
Optimal dilutions for each application to be determined by the researcher |
Ncbi Official Symbol |
CD86 |
Ncbi Official Full Name |
CD86 molecule |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P42081 |
Background |
CD86 is a receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. [UniProt] |
1st Image Caption |
Formalin-fixed, paraffin-embedded human melanoma stained with CD86 antibody (SPM600). |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.