Item no. |
PRS-33-331-100ug |
Manufacturer |
ProSci
|
Amount |
100 ug |
Category |
|
Type |
Antibody Monoclonal |
Format |
Liquid |
Applications |
FC, IF, IHC-P |
Specific against |
Human |
Host |
Mouse |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Alias |
Chromogranin-A, CgA, Pituitary secretory protein I, SP-I, Vasostatin-1, Vasostatin I, Vasostatin-2, Vasostatin II, EA-92, ES-43, Pancreastatin, SS-18, WA-8, WE-14, LF-19, AL-11, GV-19, GR-44, ER-37, CHGA |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer, Neuroscience, Signal Transduction |
Immunogen |
Recombinant human protein was used as the immunogen for the Chromogranin A antibody. |
Applications |
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Immunofluorescence: 1-2 ug/ml Immunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1) Prediluted format : incubate for 30 min at RT (2) Optimal dilution of the Chromogranin A antibody should be determined by the researcher. 1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Specificity |
Does not react with rat |
Purification |
Protein G affinity chromatography |
Clonality |
Monoclonal |
Clone |
CGA/413 |
Isotype |
IgG2b, kappa |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.1 mg/ml BSA and 0.05% sodium azide |
Concentration |
0.2 mg/mL |
Storage Conditions |
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
CHGA |
Ncbi Gene Id # |
1113 |
User Note |
Optimal dilutions for each application to be determined by the researcher |
Ncbi Official Symbol |
CHGA |
Ncbi Official Full Name |
chromogranin A (parathyroid secretory protein 1) |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P10645 |
Background |
Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin. |
1st Image Caption |
Formalin-fixed, paraffin-embedded human pheochromocytoma stained with Chromogranin A antibody (CGA/413) |
2nd Image Caption |
Formalin-fixed, paraffin-embedded human parathyroid stained with Chromogranin A antibody (CGA/413) |
3rd Image Caption |
Formalin-fixed, paraffin-embedded human pancreas stained with Chromogranin A antibody (CGA/413) |
4th Image Caption |
Formalin-fixed, paraffin-embedded human adrenal gland stained with Chromogranin A antibody (CGA/413) |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.