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BrdU Antibody [BRD494]

BrdU Antibody [BRD494]

Item no.	PRS-33-238-100ug
Manufacturer	ProSci
Amount	100 ug
Category	Antibodies Cell Biology Cell Status Apoptosis Flow Cytometry Immunohistochemistry Immunofluorescence Primary Antibodies
Type	Antibody Monoclonal
Format	Liquid
Applications	FC, IF, IHC
Specific against	other
Host	Mouse
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Available
Shipping	blue ice or RT
By Research Area	Cell Cycle
Immunogen	BrdU / Bromodeoxyuridine conjugated to KLH was used as the immunogen for this antibody.
Applications	Flow Cytometry: 0.5-1 ug/million cells IF: 0.5-1 ug/ml IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bromodeoxyuridine / BrdU antibody to be titered up or down for optimal performance. 1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37oC. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Purification	Protein G affinity chromatography
Clonality	Monoclonal
Clone	BRD494
Isotype	IgG1
Conjugate	Unconjugated
Buffer	PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration	0.2 mg/mL
Storage Conditions	Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Gene Id #	Not Applicable
User Note	Optimal dilutions for each application to be determined by the researcher
Background	This antibody reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to Bromodeoxyuridine / BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
1st Image Caption	IHC staining of mouse intestine tissue (10X) with BrdU antibody (BRD494).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

available

Delivery expected until 9/6/2024