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ACE2 Antibody

ACE2 Antibody

Item no.	PRS-3227-0.1mg
Manufacturer	ProSci
Amount	0.1 mg
Category	Oncology Neuroscience Aging & Neurological Disorders Neural Signaling Antibodies Immunohistochemistry Western Blot ELISA Immunofluorescence Primary Antibodies By Organ Testicular & Prostate Cancer
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IF, ELISA, IHC-P
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	ACE2 Antibody: ACEH, Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, ACEH, SARS-CoV receptor, SARS-CoV-2 receptor
Available
Shipping	blue ice or RT
By Research Area	Infectious Disease
Homology	Predicted species reactivity based on immunogen sequence: Bovine: (86%)
Immunogen	Anti-ACE2 antibody (3227) was raised against a peptide corresponding to 14 amino acids near the amino terminus of human ACE2. The immunogen is located within the first 50 amino acids of ACE2.
Applications	WB: 1-2 μ g/mL; IHC: 2 μ g/mL; IF: 10 μ g/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity	Anti-ACE2 has no cross response to ACE1.
Positive Control 1	Cat. No. 1305 - Human Kidney Tissue Lysate
Positive Control 2	Cat. No. 10-401 - Human Kidney Tissue Slide
Positive Control 3	Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 4	Cat. No. 1313 - Human Testis Tissue Lysate
Predicted Molecular Weight	Predicted: 93kD Observed: 130 kD (7 N-linked glycosylation)
Purification	ACE2 Antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	ACE2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	ACE2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Related Products	Cat. No. 3227P – ACE2 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	ACE2
Accession #	NP_068576
Protein Gi #	11225609
Ncbi Gene Id #	59272
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	ACE2
Ncbi Official Full Name	angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
Ncbi Organism	Homo sapiens
Swissprot #	Q9BYF1
Background	ACE2 Antibody: Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently. ACE2 has also been shown to be a functional receptor of the SARS coronavirus. Homology modeling shows 2019-nCoV has a similar receptor-binding domain structure as SARS-CoV, which suggests COVID-19 (2019-nCoV) may use ACE2 as a receptor in humans for infection. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function.
Background References 1	Donoghue et al. Circ. Res. 2000; 87:1-9.
Background References 2	Tipnis et al. J Biol. Chem. 2000; 275:33238-43.
Background References 3	Li et al. Nature 2003; 426:450-4.
Background References 4	Lu et al. The Lancet 2020 (published online).
Background References 5	Crackower et al. Nature 2002; 417:822-8.
1st Image Caption	Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Tissues Loading: 15 μ g of lysates per lane.Antibodies: ACE2, 3215 (2 μ g/mL), ACE2, 3217 (2 μ g/mL), ACE2, 3227 (2 μ g/mL) and beta-actin 3779 (1 μ g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
2nd Image Caption	Figure 2 Western Blot Validation in Human Tissues Loading: 15 μ g of lysates per lane.Antibodies: ACE2, 3227 (1 μ g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
3rd Image Caption	Figure 3 Western Blot Validation in Mouse Tissues Loading: 15 μ g of lysates per lane.Antibodies: ACE2, 3227 (2 μ g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
4th Image Caption	Figure 4 Western Blot Validation in Rat Thymus Tissue Loading: 15 μ g of lysates per lane.Antibodies: ACE2, 3227 (2 μ g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
5th Image Caption	Figure 5 Immunohistochemistry Validation of ACE2 in Human Kidney Tissue Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ACE2 antibody (3227) at 2 μ g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
6th Image Caption	Figure 6 Immunofluorescence Validation of ACE2 in Human Testis Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed human testis tissue labeling ACE-2 with 3227 at 20 μ g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
7th Image Caption	Figure 7 Immunofluorescence Validation of ACE2 in Human Lung Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung tissue labeling ACE-2 with 3227 at 20 μ g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
8th Image Caption	Figure 8 Immunofluorescence Validation of ACE2 in Mouse Lung Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung tissue labeling ACE-2 with 3227 at 20 μ g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
9th Image Caption	Figure 9 Immunofluorescence Validation of ACE2 in Rat Lung Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed rat lung tissue labeling ACE-2 with 3227 at 20 μ g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
10th Image Caption	Figure 10 Immunofluorescence Validation of ACE2 in Human Kidney Cells Immunofluorescent analysis of 4% paraformaldehyde-fixed human kidney cells labeling ACE2 with 3227 at 10 μ g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
11th Image Caption	Figure 11 Immunofluorescence Validation of ACE2 In Caco2 Cells Immunofluorescent analysis of 4% paraformaldehyde-fixed Caco2 cells labeling ACE2 with 3227 at 20 μ g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). Image showing membrane staining on Caco2 cells.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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