Manufacturer |
ProSci
|
Category |
|
Type |
Antibody Primary |
Specific against |
Mouse |
Applications |
WB, ELISA |
Amount |
0.1 mL |
Host |
Mouse |
Item no. |
PRS-32-195-0.1mL |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Alias |
ASPL, RCC17, TUG, UBXD9, UBXN9, Alveolar soft part sarcoma chromosomal region candidate gene 1 proteinTUG, ASPL, ASPS, ASPCR1 |
Shipping |
blue ice or RT |
By Research Area |
Growth Factor |
Immunogen |
Ni-NTA purified truncated recombinant TUG expressed in E. Coli strain BL21 (DE3). |
Applications |
Western Blot:1:500 - 1:2, 000 ELISA:Propose dilution 1:10, 000. Determining optimal working dilutions by titration test. |
Purification |
Antibody is purified by protein A affinity chromatography and resuspended in PBS with 0.2%BSA, 0.09% sodium azide. |
Clonality |
Monoclonal |
Clone |
4A11A6G11 |
Isotype |
IgG1 |
Conjugate |
Unconjugated |
Buffer |
Purified antibody in PBS containing 0.03% sodium azide. |
Storage Conditions |
Mouse TUG monoclonal antibody can be stored at -20˚ C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
ASPSCR1 |
Accession # |
Q9BZE9 |
Protein Gi # |
74717746 |
Ncbi Gene Id # |
79058 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
ASPSCR1 |
Ncbi Official Full Name |
alveolar soft part sarcoma chromosome region, candidate 1 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q9BZE9 |
Background |
The TUG protein contains a UBX domain, for GLUT4. In truncated form, TUG acts in a dominant-negative manner to inhibit insulin-stimulated GLUT4 redistribution in Chinese hamster ovary cells and 3T3-L1 adipocytes. Full-length TUG forms a complex specifically with GLUT4. In 3T3-L1 adipocytes, this complex is present in unstimulated cells and is largely disassembled by insulin. Endogenous TUG is localized with the insulin-mobilizable pool of GLUT4 in unstimulated 3T3-L1 adipocytes, and is not mobilized to the plasma membrane by insulin. |
Background References 1 |
Nature 2003, 425:727 - 733. |
1st Image Caption |
Western blot analysis using Mouse TUG monoclonal antibody against NIH / 3T3 cell lysate. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.