Item no. |
PRS-32-112-0.1mL |
Manufacturer |
ProSci
|
Amount |
0.1 mL |
Category |
|
Type |
Antibody Primary |
Applications |
WB, ELISA |
Specific against |
Human |
Host |
Mouse |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
G3A, NG20, HSPC336, Protein G3a, Apo-M, G3a, apo-M |
Available |
|
Shipping |
blue ice or RT |
Immunogen |
Ni-NTA purified full-length recombinant ApoM.trx expressed in E. Coli strain BL21 (DE3). |
Applications |
Western Blot:1:500 - 1:2, 000 IF:1:200 - 1:1, 000 ELISA:Propose dilution 1:10, 000. Determining optimal working dilutions by titration test. |
Purification |
Antibody is purified by protein A affinity chromatography. |
Clonality |
Monoclonal |
Clone |
8F12C6B8 |
Isotype |
IgG1 & IgG2b |
Conjugate |
Unconjugated |
Buffer |
Purified antibody in PBS containing 0.03% sodium azide. |
Storage Conditions |
ApoM monoclonal antibody can be stored at -20˚ C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
APOM |
Accession # |
O95445 |
Protein Gi # |
17370872 |
Ncbi Gene Id # |
55937 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
APOM |
Ncbi Official Full Name |
apolipoprotein M |
Ncbi Organism |
Homo sapiens |
Swissprot # |
O95445 |
Background |
ApoM(apolipoprotein M, also designated G3a or NG20), with 188-amino acid protein(about 21 kDa), is an apolipoprotein and member of the lipocalin protein family.The Apo-proteins are involved in the specific binding of cellular receptors, the regulation of lipolytic enzymes, and the process of lipid exchange. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport.The N-terminal region of Apo-M contains hydrophobic residues that may promote association with the phospholipid layer of lipoprotein particles. In vitro, Apo-M is glycosylated when translated in the presence of microsomes, and remains associated with the microsomes after carbonate treatment.Apo-M is expressed in liver and kidney, and is secreted into the bloodstream in HDLs, and also found in triglyceride-rich lipoproteins and LDLs. |
Background References 1 |
Xu, N. & Dahlback, B. 1999 J. Biol. Chem. 274:31286. |
Background References 2 |
Duan J, Dahlback B, Villoutreix BO.FEBS Lett. 2001 Jun 15; 499(1-2):127-32. |
Background References 3 |
Xu, N., Nilsson-Ehle, P. & Ahren, B. 2004. J. Nutr.Biochem. 15 (10):579-582. |
Background References 4 |
Zhang, X.Y. , et al.2004. Acta Histochem. 106 (2):123-128 |
1st Image Caption |
Western blot analysis using ApoM monoclonal antibody against human serum. |
2nd Image Caption |
Immunofluorescense analysis of L - 02 cells showing cytomembrane localization using ApoM.trx antibody. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.