Item no. |
PRS-30-584-100ul |
Manufacturer |
ProSci
|
Amount |
100 ul |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, ELISA |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
SIGLEC12, FLJ38600, S2V, SIGLECL1, SLG, Siglec-12, Siglec-L1, Siglec-XII |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Membrane , Signal Transduction |
Immunogen |
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SIGLEC12. |
Applications |
SIGLEC12 antibody can be used for detection of SIGLEC12 by ELISA at 1:1562500. SIGLEC12 antibody can be used for detection of SIGLEC12 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000. |
Positive Control 1 |
Cat. No. 1211 - HepG2 Cell Lysate |
Predicted Molecular Weight |
63 kDa |
Purification |
Antibody is purified by peptide affinity chromatography method. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Concentration |
batch dependent |
Storage Conditions |
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store SIGLEC12 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles. |
Disclaimer |
This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
SIGLEC12 |
Accession # |
NP_443729 |
Protein Gi # |
16506828 |
Ncbi Gene Id # |
89858 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
SIGLEC12 |
Ncbi Official Full Name |
sialic acid binding Ig-like lectin 12 (gene/pseudogene) |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q96PQ1 |
Background |
Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily. They mediate protein-carbohydrate interactions by selectively binding to different sialic acid moieties present on glycolipids and glycoproteins. SIGLEC12 is a member of the SIGLEC3-like subfamily of SIGLECs. SIGLEC12, upon tyrosine phosphorylation, has been shown to recruit the Src homology 2 domain-containing protein-tyrosine phosphatases SHP1 and SHP2. It has been suggested that the protein is involved in the negative regulation of macrophage signaling by functioning as an inhibitory receptor.Western blots using four different antibodies against four unique regions of this protein target confirm the same apparent molecular weight in our tests.Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily. They mediate protein-carbohydrate interactions by selectively binding to different sialic acid moieties present on glycolipids and glycoproteins. This gene encodes a member of the SIGLEC3-like subfamily of SIGLECs. Members of this subfamily are characterized by an extracellular V-set immunoglobulin-like domain followed by two C2-set immunoglobulin-like domains, and the cytoplasmic tyrosine-based motifs ITIM and SLAM-like. The encoded protein, upon tyrosine phosphorylation, has been shown to recruit the Src homology 2 domain-containing protein-tyrosine phosphatases SHP1 and SHP2. It has been suggested that the protein is involved in the negative regulation of macrophage signaling by functioning as an inhibitory receptor. This gene is located in a cluster with other SIGLEC3-like genes on 19q13.4. Alternatively spliced transcript variants encoding distinct isoforms have been described for this gene. |
Background References 1 |
Angata, T., (2001) J. Biol. Chem. 276 (43), 40282-40287. |
1st Image Caption |
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.