Item no. |
PRS-30-427-100ul |
Manufacturer |
ProSci
|
Amount |
100 ul |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, ELISA |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
MFNG, |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Signal Transduction |
Immunogen |
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MFNG. |
Applications |
MFNG antibody can be used for detection of MFNG by ELISA at 1:62500. MFNG antibody can be used for detection of MFNG by western blot at 0.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000. |
Positive Control 1 |
Cat. No. 1211 - HepG2 Cell Lysate |
Predicted Molecular Weight |
36 kDa |
Purification |
Antibody is purified by peptide affinity chromatography method. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Concentration |
batch dependent |
Storage Conditions |
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store MFNG antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles. |
Disclaimer |
This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
MFNG |
Accession # |
NP_002396 |
Protein Gi # |
21536461 |
Ncbi Gene Id # |
4242 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
MFNG |
Ncbi Official Full Name |
MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase |
Ncbi Organism |
Homo sapiens |
Swissprot # |
O00587 |
Background |
MFNG is one of the evolutionarily conserved secreted proteins that act in the Notch receptor pathway to demarcate boundaries during embryonic development. Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene is a member of the fringe gene family which also includes Radical and Lunatic fringe. They all encode evolutionarily conserved secreted proteins that act in the Notch receptor pathway to demarcate boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, fringe proteins have a fucose-specific beta1, 3 N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
Background References 1 |
Veeraraghavalu, K., (2004) J. Virol. 78 (16), 8687-8700. |
1st Image Caption |
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.