Item no. |
PRS-30-346-100ul |
Manufacturer |
ProSci
|
Amount |
100 ul |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, ELISA |
Specific against |
Human, Rat, Canine |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
DKK1, DKK-1, SK |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer , Signal Transduction , Growth Factor |
Immunogen |
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DKK1. |
Applications |
DKK1 antibody can be used for detection of DKK1 by ELISA at 1:312500. DKK1 antibody can be used for detection of DKK1 by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000. |
Positive Control 1 |
Cat. No. 1211 - HepG2 Cell Lysate |
Predicted Molecular Weight |
26 kDa |
Purification |
Antibody is purified by protein A chromatography method. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Concentration |
batch dependent |
Storage Conditions |
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store DKK1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles. |
Disclaimer |
This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
DKK1 |
Accession # |
NP_036374 |
Protein Gi # |
7110719 |
Ncbi Gene Id # |
22943 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
DKK1 |
Ncbi Official Full Name |
dickkopf WNT signaling pathway inhibitor 1 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
O94907 |
Background |
DKK1 is a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. |
Background References 1 |
Ai, M., (2005) Mol. Cell. Biol. 25 (12), 4946-4955. |
1st Image Caption |
Antibody used in WB on Human cell lines at: 1:1000 (Lane 1: 30ug human PLC/PRF5 cell lysate, Lane 2: 30ug DKK1 PLC/PRF5 cell lysate ). |
2nd Image Caption |
Antibody used in WB on Human HepG2 at 2.5 ug/ml. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.