Comparison

DKK1 Antibody

Item no. PRS-30-346-100ul
Manufacturer ProSci
Amount 100 ul
Category
Type Antibody Polyclonal
Format Liquid
Applications WB, ELISA
Specific against Human, Rat, Canine
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias DKK1, DKK-1, SK
Available
Shipping
blue ice or RT
By Research Area
Cancer , Signal Transduction , Growth Factor
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DKK1.
Applications
DKK1 antibody can be used for detection of DKK1 by ELISA at 1:312500. DKK1 antibody can be used for detection of DKK1 by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000.
Positive Control 1
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight
26 kDa
Purification
Antibody is purified by protein A chromatography method.
Clonality
Polyclonal
Conjugate
Unconjugated
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration
batch dependent
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store DKK1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Disclaimer
This product is for research use only.
Modifications
None
Ncbi Official Symbol
DKK1
Accession #
NP_036374
Protein Gi #
7110719
Ncbi Gene Id #
22943
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol
DKK1
Ncbi Official Full Name
dickkopf WNT signaling pathway inhibitor 1
Ncbi Organism
Homo sapiens
Swissprot #
O94907
Background
DKK1 is a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
Background References 1
Ai, M., (2005) Mol. Cell. Biol. 25 (12), 4946-4955.
1st Image Caption
Antibody used in WB on Human cell lines at: 1:1000 (Lane 1: 30ug human PLC/PRF5 cell lysate, Lane 2: 30ug DKK1 PLC/PRF5 cell lysate ).
2nd Image Caption
Antibody used in WB on Human HepG2 at 2.5 ug/ml.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ul
Available: In stock
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