Item no. |
PRS-29-802-100ul |
Manufacturer |
ProSci
|
Amount |
100 ul |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IHC, ELISA |
Specific against |
Human, Mouse, Canine |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
HDAC9, DKFZp779K1053, HD7, HDAC, HDAC7, HDAC7B, HDAC9B, HDAC9FL, HDRP, KIAA0744, MITR, HD9, HD7b |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Transcription , Cell Cycle , Signal Transduction , Immunology |
Immunogen |
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HDAC9. |
Applications |
HDAC9 antibody can be used for detection of HDAC9 by ELISA at 1:1562500. HDAC9 antibody can be used for detection of HDAC9 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000. |
Positive Control 1 |
A172 Cell Lysate |
Predicted Molecular Weight |
65 kDa |
Purification |
Antibody is purified by protein A chromatography method. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Concentration |
batch dependent |
Storage Conditions |
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store HDAC9 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles. |
Disclaimer |
This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
HDAC9 |
Accession # |
NP_055522 |
Protein Gi # |
7662280 |
Ncbi Gene Id # |
9734 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
HDAC9 |
Ncbi Official Full Name |
histone deacetylase 9 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
A7E2F3 |
Background |
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. HDAC9 has sequence homology to members of the histone deacetylase family. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. HDAC9 may play a role in hematopoiesis.Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. Multiple alternatively spliced transcripts have been described for this gene but the full-length nature of some of them has not been determined. |
Background References 1 |
Han, A., (2005) J. Mol. Biol. 345 (1), 91-102. |
1st Image Caption |
Antibody used in IHC on Human kidney. |
2nd Image Caption |
Antibody used in WB on Human A172 at 1.25 ug/ml. |
3rd Image Caption |
Antibody used in CHIP on HCT116 Cells. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.