Item no. |
PRS-29-577-100ul |
Manufacturer |
ProSci
|
Amount |
100 ul |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IHC, ELISA |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
MUC1, CD227, EMA, PEM, PUM, mucin, MCD, KL-6, MAM6, MCKD, PEMT, H23AG, MCKD1, MUC-1, ADMCKD, ADMCKD1, CA 15-3, MUC-1/X, MUC1/ZD, MUC-1/SEC |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer |
Immunogen |
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MUC1. |
Applications |
MUC1 antibody can be used for detection of MUC1 by ELISA at 1:312500. MUC1 antibody can be used for detection of MUC1 by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000. |
Positive Control 1 |
Cat. No. 1205 - Jurkat Cell Lysate |
Predicted Molecular Weight |
22 kDa, 28 kDa, 28 kDa, 16 kDa, 17 kDa, 30 kDa |
Purification |
Antibody is purified by protein A chromatography method. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Concentration |
batch dependent |
Storage Conditions |
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store MUC1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles. |
Disclaimer |
This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
MUC1 |
Accession # |
NP_001037855 |
Protein Gi # |
113206024 |
Ncbi Gene Id # |
4582 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
MUC1 |
Ncbi Official Full Name |
mucin 1, cell surface associated |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q7Z552 |
Background |
MUC1 is a membrane bound, glycosylated phosphoprotein. The protein is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. It also includes a 20 aa variable number tandem repeat (VNTR) domain, with the number of repeats varying from 20 to 120 in different individuals. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. |
1st Image Caption |
Antibody used in IHC on Human stomach at 1:500. |
2nd Image Caption |
Antibody used in IHC on Pig stomach at 1:500. |
3rd Image Caption |
Antibody used in WB on Human Jurkat 2.5 ug/ml. |
4th Image Caption |
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.