Item no. |
PRS-29-453-100ul |
Manufacturer |
ProSci
|
Amount |
100 ul |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, ELISA |
Specific against |
Human, Mouse, Rat, Canine |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
PCBP4, CBP, LIP4, MCG10 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
RNA Binding , Cancer |
Immunogen |
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PCBP4. |
Applications |
PCBP4 antibody can be used for detection of PCBP4 by ELISA at 1:62500. PCBP4 antibody can be used for detection of PCBP4 by western blot at 0.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000. |
Positive Control 1 |
Cat. No. 1205 - Jurkat Cell Lysate |
Predicted Molecular Weight |
47 kDa, 41 kDa, 38 kDa, 41 kDa |
Purification |
Antibody is purified by peptide affinity chromatography method. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Concentration |
batch dependent |
Storage Conditions |
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PCBP4 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles. |
Disclaimer |
This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
PCBP4 |
Accession # |
NP_065151 |
Protein Gi # |
291575150 |
Ncbi Gene Id # |
57060 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
PCBP4 |
Ncbi Official Full Name |
poly(rC) binding protein 4 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q9GZT1 |
Background |
PCBP4 is a member of the KH-domain protein subfamily. Proteins of this subfamily, also referred to as alpha-CPs, bind to RNA with a specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. This gene is induced by the p53 tumor suppressor, and the protein can suppress cell proliferation by inducing apoptosis and cell cycle arrest in G (2)-M.This gene encodes a member of the KH-domain protein subfamily. Proteins of this subfamily, also referred to as alpha-CPs, bind to RNA with a specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. This gene is induced by the p53 tumor suppressor, and the encoded protein can suppress cell proliferation by inducing apoptosis and cell cycle arrest in G (2)-M. This gene's protein is found in the cytoplasm, yet it lacks the nuclear localization signals found in other subfamily members. Multiple alternatively spliced transcript variants have been described, but the full-length nature for only some has been determined. |
Background References 1 |
Chkheidze, A.N. (2003) Mol. Cell. Biol. 23 (23), 8405-8415. |
1st Image Caption |
Antibody used in WB on Human Jurkat 0.2-1 ug/ml. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.