Item no. |
PRS-29-019-100ul |
Manufacturer |
ProSci
|
Amount |
100 ul |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, ELISA |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
TRIM35, HLS5, MAIR |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Transcription , Cancer |
Immunogen |
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TRIM35. |
Applications |
TRIM35 antibody can be used for detection of TRIM35 by ELISA at 1:12500. TRIM35 antibody can be used for detection of TRIM35 by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000. |
Positive Control 1 |
Cat. No. 1211 - HepG2 Cell Lysate |
Predicted Molecular Weight |
57 kDa, 57 kDa |
Purification |
Antibody is purified by protein A chromatography method. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Concentration |
batch dependent |
Storage Conditions |
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store TRIM35 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles. |
Disclaimer |
This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
TRIM35 |
Accession # |
NP_055881 |
Protein Gi # |
25777692 |
Ncbi Gene Id # |
23087 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
TRIM35 |
Ncbi Official Full Name |
tripartite motif containing 35 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q9UPQ4 |
Background |
TRIM35 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. TRIM35 may play a role as a tumor suppressor, and is implicated in the cell death mechanism |
Background References 1 |
Lalonde, J.P., (2004) J. Biol. Chem. 279 (9), 8181-8189. |
1st Image Caption |
Antibody used in WB on Human HepG2 at 2.5 ug/ml. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.