Item no. |
PRS-28-535-100ul |
Manufacturer |
ProSci
|
Amount |
100 ul |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, ELISA |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
MCM2, BM28, CCNL1, CDCL1, cdc19, D3S3194, MITOTIN |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cell Cycle , Cancer |
Immunogen |
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MCM2. |
Applications |
MCM2 antibody can be used for detection of MCM2 by ELISA at 1:312500. MCM2 antibody can be used for detection of MCM2 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000. |
Positive Control 1 |
Cat. No. 1205 - Jurkat Cell Lysate |
Predicted Molecular Weight |
99 kDa |
Purification |
Antibody is purified by protein A chromatography method. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Concentration |
batch dependent |
Storage Conditions |
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store MCM2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles. |
Disclaimer |
This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
MCM2 |
Accession # |
NP_004517 |
Protein Gi # |
33356547 |
Ncbi Gene Id # |
4171 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
MCM2 |
Ncbi Official Full Name |
minichromosome maintenance complex component 2 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P49736 |
Background |
The protein encoded by MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. |
Background References 1 |
Yu, Z., et al., (2004) J. Mol. Biol. 340 (5), 1197-1206. |
1st Image Caption |
Antibody used in WB on Human Jurkat 1.25 ug/ml. |
2nd Image Caption |
Antibody used in WB on mouse neural stem at 1:1000. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.