Item no. |
PRS-28-177-100ul |
Manufacturer |
ProSci
|
Amount |
100 ul |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IHC, ELISA |
Specific against |
Human, Mouse, Rat, Canine |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
KEAP1, INrf2, KIAA0132, KLHL19, MGC10630, MGC1114, MGC20887, MGC4407, MGC9454 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Transcription , Cancer |
Immunogen |
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human KEAP1. |
Applications |
KEAP1 antibody can be used for detection of KEAP1 by ELISA at 1:1562500. KEAP1 antibody can be used for detection of KEAP1 by western blot at 1.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000. |
Positive Control 1 |
Cat. No. 1211 - HepG2 Cell Lysate |
Predicted Molecular Weight |
70 kDa, 70 kDa |
Purification |
Antibody is purified by protein A chromatography method. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Concentration |
batch dependent |
Storage Conditions |
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store KEAP1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles. |
Disclaimer |
This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
KEAP1 |
Accession # |
NP_987096 |
Protein Gi # |
45269145 |
Ncbi Gene Id # |
9817 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
KEAP1 |
Ncbi Official Full Name |
kelch-like ECH-associated protein 1 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q14145 |
Background |
KEAP1 contains KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase.This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. |
Background References 1 |
Padmanabhan, B., (2006) Mol. Cell 21 (5), 689-700. |
1st Image Caption |
Antibody used in IHC on Human kidney. |
2nd Image Caption |
Antibody used in WB on Human HepG2 at 1 ug/ml. |
3rd Image Caption |
Antibody used in WB on HepG2 at 1.25 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ). |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.