Item no. |
PRS-25-132-100ul |
Manufacturer |
ProSci
|
Amount |
100 ul |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, ELISA |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
ING3, Eaf4, FLJ20089, ING2, p47ING3, MEAF4 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer , Homeostasis |
Immunogen |
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ING3. |
Applications |
ING3 antibody can be used for detection of ING3 by ELISA at 1:62500. ING3 antibody can be used for detection of ING3 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000. |
Positive Control 1 |
Cat. No. 1211 - HepG2 Cell Lysate |
Predicted Molecular Weight |
47 kDa |
Purification |
Antibody is purified by peptide affinity chromatography method. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Concentration |
batch dependent |
Storage Conditions |
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store ING3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles. |
Disclaimer |
This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
ING3 |
Accession # |
NP_061944 |
Protein Gi # |
38201655 |
Ncbi Gene Id # |
54556 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
ING3 |
Ncbi Official Full Name |
inhibitor of growth family, member 3 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q9NXR8 |
Background |
ING3 is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This gene can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. Overexpression of this gene has been shown to inhibit cell growth and induce apoptosis. Allelic loss and reduced expression of this gene were detected in head and neck cancers.The protein encoded by this gene is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This gene can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. Overexpression of this gene has been shown to inhibit cell growth and induce apoptosis. Allelic loss and reduced expression of this gene were detected in head and neck cancers. Two alternatively spliced transcript variants encoding different isoforms have been observed. |
Background References 1 |
Gunduz, M., (2008) Cancer Sci. 99 (3), 531-538. |
1st Image Caption |
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml. |
2nd Image Caption |
Antibody used in IHC on Human Placenta at 5 ug/ml. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.